Mettenleiter T C, Zsak L, Kaplan A S, Ben-Porat T, Lomniczi B
Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
J Virol. 1987 Dec;61(12):4030-2. doi: 10.1128/JVI.61.12.4030-4032.1987.
The virulence of deletion mutants of pseudorabies virus defective in the expression of glycoprotein gI, gp63, or both was tested in 1-day-old chickens and young pigs. In the absence of expression of gI, the virulence of a fully virulent laboratory strain, PrV(Ka), for 1-day-old chickens was reduced approximately fourfold. Inactivation of glycoprotein gp63 appeared also to affect the virulence of PrV(Ka) only slightly, as did inactivation of both gI and gp63. The level of reduction in virulence, however, was considerably more marked in Bartha 43/25aB4, a less virulent virus strain. Inactivation of the expression of gI in Bartha 43/25aB4 reduced virulence for chickens at least 100-fold. The results obtained when the virulence of the mutants for pigs was determined were compatible with those obtained for chickens. These results indicate that gI plays a role in virulence, but that it does so in conjunction with at least one other viral function (a function that is defective in Bartha 43/25aB4).
在1日龄雏鸡和幼猪中测试了糖蛋白gI、gp63或两者表达缺陷的伪狂犬病病毒缺失突变体的毒力。在缺乏gI表达的情况下，完全毒力的实验室毒株PrV(Ka)对1日龄雏鸡的毒力降低了约四倍。糖蛋白gp63的失活似乎也仅轻微影响PrV(Ka)的毒力，gI和gp63两者失活时也是如此。然而，在毒力较弱的病毒株Bartha 43/25aB4中，毒力降低的程度更为显著。Bartha 43/25aB4中gI表达的失活使对鸡的毒力降低了至少100倍。测定突变体对猪的毒力时获得的结果与对鸡获得的结果一致。这些结果表明，gI在毒力中起作用，但它是与至少一种其他病毒功能（一种在Bartha 43/25aB4中存在缺陷 的功能）共同发挥作用的。
