Arase S, Shikiji T, Uchida N, Katoh S, Fujie T, Urano Y
Department of Dermatology, School of Medicine, University of Tokushima, Japan.
Skin Pharmacol. 1994;7(1-2):12-5. doi: 10.1159/000211267.
When plucked follicles were cultured together with isolated dermal papillae in a collagen gel matrix, outer root sheath cells (ORSCs) grew more rapidly and actively than without the papillae. Cultured dermal papilla cells also activated the colony growth of ORSCs in vitro. These results may suggest the existence of some papilla-derived factor(s) activating the growth of ORSCs. In cultures of excised whole follicles whose dermal papilla had been removed, epithelial cells of bulb matrix origin grew out from the bulbous portion, and formed spikes. When a dermal papilla was implanted close to the follicle, the spikes elongated toward the papilla, and finally reached and surrounded it. This finding suggests that dermal papillae may produce some factor(s) attracting epithelial cells of hair bulb origin. In cultures of excised whole follicles, when the dermal papilla remained originally positioned in contact with the hair bulb matrix, the hair and follicle elongated for more than 1 week. But when the dermal papilla was detached from the bulb matrix, the matrix cells proliferated into the gel and formed a hair-follicle-like structure (folliculoid). In cultures of excised whole follicles whose papilla-matrix junction had been damaged by dispase, elongation of the hair and follicle was almost completely suppressed. Thus, the attachment of the dermal papilla to the bulb matrix appears to be necessary for normal hair and follicle growth.
当将拔除的毛囊与分离的真皮乳头在胶原凝胶基质中共同培养时，外根鞘细胞（ORSCs）的生长比没有乳头时更快且更活跃。培养的真皮乳头细胞在体外也能激活ORSCs的集落生长。这些结果可能提示存在一些由乳头衍生的、激活ORSCs生长的因子。在去除了真皮乳头的完整毛囊的培养物中，球部基质来源的上皮细胞从球部生长出来，并形成尖状物。当将一个真皮乳头植入靠近毛囊的位置时，尖状物会朝着乳头伸长，最终到达并包围它。这一发现表明真皮乳头可能产生一些吸引毛球来源上皮细胞的因子。在完整毛囊的培养物中，当真皮乳头保持最初与毛球基质接触的位置时，毛发和毛囊会伸长超过1周。但当真皮乳头与球部基质分离时，基质细胞会增殖进入凝胶并形成类似毛囊的结构（类毛囊）。在经dispase破坏了乳头 - 基质连接处的完整毛囊的培养物中，毛发和毛囊的伸长几乎被完全抑制。因此，真皮乳头与球部基质的附着似乎是正常毛发和毛囊生长所必需的。
