Arase S, Sadamoto Y, Katoh S, Urano Y, Takeda K
Department of Dermatology, School of Medicine, University of Tokushima, Japan.
J Dermatol. 1990 Nov;17(11):667-76. doi: 10.1111/j.1346-8138.1990.tb03009.x.
Human hair follicles, either alone or in combination with dermal papillae, were cultured in a collagen matrix. When plucked hair follicles were cultured alone, spike-like structures composed of outer root sheath cells started growing around the follicle and then radiated into the gel. When isolated dermal papillae were embedded close to the follicles, spikes started growing earlier and grew more rapidly than without the papillae. In cultures of excised follicles from which the dermal papilla had been removed, epithelial cells (possibly hair bulb cells) started growing out from the bulbous portion and then also formed spikes. In the presence of a papilla, the spikes elongated toward the papilla, finally reaching and surrounding it. These findings suggest that dermal papilla cells produce a factor(s) that enhances growth of follicular epithelial cells and also attracts those cells. In cultures of whole excised follicles, two major characteristic patterns of cellular growth were recognized. When the dermal papilla remained inside the bulb in contact with the hair bulb matrix, the hair matrix cells proliferated and differentiated in the normal manner, resulting in elongation of the hair shaft and follicle. But when the papilla was detached from the hair bulb matrix, epithelial cells proliferated from the bulbous portion and finally formed hair follicle-like structures. Thus, attachment of the dermal papilla to the hair bulb matrix in the bulbous portion appears to be necessary for growth of the hair and follicle in the normal manner. Our model may be useful for examining the interaction between follicular epithelial cells and dermal papillae and for studying the growth of hair and follicles in vitro.
人毛囊单独或与真皮乳头联合培养于胶原基质中。当拔除的毛囊单独培养时，由外根鞘细胞组成的穗状结构开始在毛囊周围生长，然后向凝胶中呈放射状生长。当分离的真皮乳头靠近毛囊嵌入时，穗状结构比没有乳头时更早开始生长且生长更快。在去除真皮乳头的切除毛囊培养物中，上皮细胞（可能是毛球细胞）开始从球部生长出来，然后也形成穗状结构。在有乳头存在的情况下，穗状结构朝着乳头伸长，最终到达并包围乳头。这些发现表明真皮乳头细胞产生一种因子，该因子可促进毛囊上皮细胞生长并吸引这些细胞。在整个切除毛囊的培养物中，识别出两种主要的细胞生长特征模式。当真皮乳头留在球部内与毛球基质接触时，毛基质细胞以正常方式增殖和分化，导致毛干和毛囊伸长。但是当乳头与毛球基质分离时，上皮细胞从球部增殖，最终形成毛囊样结构。因此，真皮乳头在球部与毛球基质的附着似乎是毛发和毛囊正常生长所必需的。我们的模型可能有助于研究毛囊上皮细胞与真皮乳头之间的相互作用以及体外毛发和毛囊的生长。
