19-去甲醛固酮在分离的大鼠肾小球旁细胞中的生物合成途径。

Biosynthetic pathway of 19-noraldosterone in isolated rat glomerulosa cells.

作者信息

Takeda Y, Miyamori I, Iki K, Yoneda T, Takeda R

机构信息

Second Department of Internal Medicine, School of Medicine, Kanazawa University, Japan.

出版信息

J Steroid Biochem Mol Biol. 1994 May;49(1):69-71. doi: 10.1016/0960-0760(94)90302-6.

DOI:10.1016/0960-0760(94)90302-6

PMID:8003441

Abstract

The biosynthetic pathway of 19-noraldosterone (19-noraldo) in isolated rat glomerulosa cells (GC) and fasciculata-reticular cells (FC) was studied by analyzing [14C]pregnenolone metabolism using HPLC and quantification by specific RIA. In GCs, 18,19-dihydroxycorticosterone was detected after 15 min incubation with [14C]pregnenolone, 18-hydroxy-19-norcorticosterone was detected after 30 min and 19-noraldo was detected after 45 min before the appearance of an aldosterone peak. These three mineralocorticoids were not detected in FCs. The results demonstrate that 19-noraldo is synthesized in GCs and then undergoes further metabolism.

摘要

通过使用高效液相色谱法（HPLC）分析[14C]孕烯醇酮代谢，并通过特异性放射免疫分析（RIA）进行定量，研究了分离的大鼠肾小球旁细胞（GC）和束状-网状细胞（FC）中19-去甲醛固酮（19-noraldo）的生物合成途径。在GC中，与[14C]孕烯醇酮孵育15分钟后检测到18,19-二羟基皮质酮，30分钟后检测到18-羟基-19-去甲皮质酮，45分钟后在醛固酮峰出现之前检测到19-noraldo。在FC中未检测到这三种盐皮质激素。结果表明，19-noraldo在GC中合成，然后进行进一步代谢。