Vita M, Smith K, Rozman D, Komel R
Institute of Biochemistry, Medical Faculty, Ljubljana, Slovenia.
J Steroid Biochem Mol Biol. 1994 May;49(1):87-92. doi: 10.1016/0960-0760(94)90305-0.
Studies of Cochliobolus lunatus m118 steroid metabolism by thin-layer chromatography, mass spectrometry and NMR spectroscopy revealed that the fungus hydroxylates progesterone at positions 7 alpha, 11 beta and 14 alpha, and oxidizes the 11 beta-hydroxy group to the ketone. The 1H NMR spectra of two of the steroid metabolites, 11 beta,14 alpha-dihydroxyprogesterone and 11-oxo-14 alpha-hydroxyprogesterone, are reported for the first time. It is still not known if all the hydroxylation reactions are performed in C. lunatus by a single, non-specific, steroid hydroxylase, structurally different from the 11 beta-hydroxylase found in higher eucaryotes, or if different forms of the enzyme are involved.
通过薄层色谱法、质谱法和核磁共振光谱法对新月弯孢霉m118甾体代谢的研究表明，该真菌可在7α、11β和14α位使孕酮羟基化，并将11β-羟基氧化为酮。首次报道了两种甾体代谢产物11β,14α-二羟基孕酮和11-氧代-14α-羟基孕酮的1H核磁共振光谱。目前尚不清楚所有羟基化反应是否由新月弯孢霉中一种结构不同于高等真核生物中发现的11β-羟化酶的单一非特异性甾体羟化酶进行，还是涉及该酶的不同形式。
