甲状旁腺激素相关蛋白基因在宫颈浸润性肿瘤中的表达
Parathyroid hormone-related protein gene expression in invasive cervical tumors.
作者信息
Dunne F P, Rollason T, Ratcliffe W A, Marshall T, Heath D A
机构信息
Department of Medicine, Queen Elizabeth Hospital, Edgbaston, Birmingham, United Kingdom.
出版信息
Cancer. 1994 Jul 1;74(1):83-9. doi: 10.1002/1097-0142(19940701)74:1<83::aid-cncr2820740115>3.0.co;2-w.
BACKGROUND
Parathyroid hormone-related protein (PTHrP) is the main tumor-derived factor responsible for the hypercalcemia of malignancy.
METHODS
Using a polyclonal serum to the 37-67 region of PTHrP, and 35S-labeled riboprobes, the authors investigated the cellular expression of PTHrP mRNA and peptide in formalin fixed, paraffin embedded sections from 16 invasive cervical tumors. In addition, the relationship among the histologic cell type, degree of differentiation, pattern of invasion, and tumor expression of PTHrP were examined.
RESULTS
PTHrP mRNA and peptide were identified in 10 of 10 and 16 of 16 tumors examined, respectively. Overall strong mRNA expression with moderate to intense intracellular staining for peptide was associated, with adenosquamous carcinoma displaying a spray pattern of invasion.
CONCLUSION
PTHrP mRNA and peptide were observed in all cervical tumors studied. Despite their high frequency of expression of PTHrP, cervical tumors seldom give rise to humoral hypercalcemia of malignancy, but the autocrine/paracrine effects of PTHrP may be important in the growth and dedifferentiation of the malignant cell population.
背景
甲状旁腺激素相关蛋白（PTHrP）是导致恶性肿瘤高钙血症的主要肿瘤衍生因子。
方法
作者使用针对PTHrP 37 - 67区域的多克隆血清和35S标记的核糖探针，研究了16例浸润性宫颈癌福尔马林固定、石蜡包埋切片中PTHrP mRNA和肽的细胞表达。此外，还检查了组织学细胞类型、分化程度、侵袭模式与PTHrP肿瘤表达之间的关系。
结果
在所检查的10例肿瘤中的10例以及16例肿瘤中的16例中分别鉴定出PTHrP mRNA和肽。总体上，mRNA强表达与肽的中度至强细胞内染色相关，腺鳞癌表现出浸润的喷射模式。
结论
在所研究的所有宫颈癌中均观察到PTHrP mRNA和肽。尽管宫颈癌中PTHrP表达频率较高，但很少引起恶性肿瘤的体液性高钙血症，不过PTHrP的自分泌/旁分泌作用可能在恶性细胞群体的生长和去分化中起重要作用。
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