Johnson R, Kaneene J B, Lloyd J W
Department of Clinical Sciences, College of Veterinary Medicine, E. Lansing, MI 48824-1316.
Comp Immunol Microbiol Infect Dis. 1994 Feb;17(1):1-13. doi: 10.1016/0147-9571(94)90002-7.
The objective of this study was to investigate the effects of bovine leukemia virus (BLV) infection in sheep. A prospective study of the serologic, hematologic, and histologic changes of sheep infected with BLV was conducted. Antibodies to BLV were detectable in the sheep 3 weeks after exposure to blood from an infected cow and persisted during a 120 week examination period, whereas all control sheep remained seronegative. There were no statistically significant differences between the leucocyte counts, lymphocyte counts, and lymphocyte percentages of the infected and control sheep during the first 120 weeks of this study. However, one sheep did develop a leukopenia and lymphopenia 95 weeks after it became infected and died of histologically-confirmed lymphosarcoma 10 days later. A lymphocyte colony assay was used to study the effects of BLV infection on colony formation by sheep lymphocytes in vitro. There was no significant difference in the number of lymphocyte colonies formed by BLV infected and control sheep. Nor was there a significant difference in the number of colonies formed by lymphocytes from the BLV infected sheep, when the autologous sheep serum was replaced with either pooled serum from the infected sheep or with pooled serum from the control sheep. BLV infection in aleukemic sheep does not appear to have an adverse affect on colony formation by lymphocytes in vitro.
本研究的目的是调查牛白血病病毒（BLV）感染对绵羊的影响。对感染BLV的绵羊的血清学、血液学和组织学变化进行了前瞻性研究。在绵羊接触来自感染牛的血液3周后可检测到BLV抗体，并且在120周的检查期内持续存在，而所有对照绵羊仍为血清阴性。在本研究的前120周内，感染绵羊和对照绵羊的白细胞计数、淋巴细胞计数和淋巴细胞百分比之间没有统计学上的显著差异。然而，一只绵羊在感染95周后出现白细胞减少和淋巴细胞减少，并在10天后死于组织学确诊的淋巴肉瘤。使用淋巴细胞集落测定法研究BLV感染对绵羊淋巴细胞体外集落形成的影响。BLV感染绵羊和对照绵羊形成的淋巴细胞集落数量没有显著差异。当用感染绵羊的混合血清或对照绵羊的混合血清替代自体绵羊血清时，来自BLV感染绵羊的淋巴细胞形成的集落数量也没有显著差异。无白血病绵羊中的BLV感染似乎对淋巴细胞体外集落形成没有不利影响。
