Hailata N, Johnson R, al-Bagdadi F, Hanash S
Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid.
Vet Immunol Immunopathol. 1995 Feb;44(3-4):211-22. doi: 10.1016/0165-2427(94)05319-n.
Proliferating cell nuclear antigen (PCNA) or cyclin (C), a major nuclear protein, has been shown to be associated with human leukemia and malignancies. PCNA protein was quantitated in this study, in lymphocytes from bovine leukemia virus (BLV) infected and non-infected sheep, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and silver staining. The PCNA mean levels in lymphocytes of BLV-infected sheep (27 months post-infection) was significantly (P < 0.001) higher than in the lymphocytes of the non-infected sheep. The mean of PCNA levels in lymphocytes of sheep, 21 days after BLV infection, showed a two-fold increase compared with the non-infected sheep. Phytohemagglutinin (PHA) (3 days) treatment of lymphocytes from the non-infected and from the BLV-infected sheep resulted in a significant (P < 0.01) increase in the mean of PCNA levels only in the non-infected sheep. The mean lymphocyte counts of the BLV-infected sheep were not significantly different from the mean counts of the non-infected sheep at the time of lymphocyte protein analysis. Thus, these findings showed, similar to human leukemia and malignancies, that high levels of PCNA were found in lymphocytes from BLV-infected sheep compared with those from the non-infected sheep, and this was independent of high cell count. Our results also suggest that PCNA protein may play a role in the process of lymphoid transformation as a result of BLV infection of sheep.
增殖细胞核抗原（PCNA）或细胞周期蛋白（C）是一种主要的核蛋白，已被证明与人类白血病和恶性肿瘤有关。在本研究中，使用二维聚丙烯酰胺凝胶电泳（2-D PAGE）和银染法对感染和未感染牛白血病病毒（BLV）的绵羊淋巴细胞中的PCNA蛋白进行了定量分析。感染BLV的绵羊（感染后27个月）淋巴细胞中的PCNA平均水平显著高于未感染绵羊（P < 0.001）。BLV感染后21天，绵羊淋巴细胞中PCNA水平的平均值相较于未感染绵羊增加了两倍。用植物血凝素（PHA）（处理3天）处理未感染和感染BLV的绵羊的淋巴细胞，结果显示仅在未感染绵羊中PCNA水平的平均值显著升高（P < 0.01）。在进行淋巴细胞蛋白质分析时，感染BLV的绵羊的平均淋巴细胞计数与未感染绵羊的平均计数没有显著差异。因此，这些发现表明，与人类白血病和恶性肿瘤相似，感染BLV的绵羊淋巴细胞中PCNA水平高于未感染绵羊，且这与高细胞计数无关。我们的结果还表明，PCNA蛋白可能在绵羊感染BLV导致的淋巴样转化过程中发挥作用。
