Hong J S, Rubinsky B
Department of Mechanical Engineering, University of California, Berkeley 94720.
Cryobiology. 1994 Apr;31(2):109-20. doi: 10.1006/cryo.1994.1015.
Normal and malignant human breast tissues were obtained from resection surgery and frozen on a directional solidification stage with controlled and uniform cooling rates. The frozen samples were freeze substituted and examined with a light microscope. It was observed that in both normal and malignant tissue, ice forms first in the connective tissue. The ice propagates along the connective tissue, which surrounds fat cells in normal adipose tissue and clumps of tightly packed malignant cells in the diseased tissue. This mode of freezing affects the osmotic response of malignant cells, causing intracellular ice crystal formation at unusually low cooling rates.
正常和恶性人类乳腺组织取自切除手术，并在具有可控且均匀冷却速率的定向凝固阶段进行冷冻。冷冻样本经过冷冻置换后，用光学显微镜进行检查。观察发现，在正常和恶性组织中，冰均首先在结缔组织中形成。冰沿着结缔组织扩散，在正常脂肪组织中结缔组织围绕着脂肪细胞，而在患病组织中结缔组织围绕着紧密堆积的恶性细胞团块。这种冷冻方式会影响恶性细胞的渗透反应，导致在异常低的冷却速率下形成细胞内冰晶。
