Pietri S, Séguin J R, d'Arbigny P D, Culcasi M
S.R.E.P., CNRS URA 1412, Université de Provence, Marseille, France.
Free Radic Biol Med. 1994 Apr;16(4):523-8. doi: 10.1016/0891-5849(94)90131-7.
To assess the development of oxidative stress in cardiac ischemia/reperfusion, the resulting depletion of plasma ascorbate was monitored by electron spin resonance spectroscopic detection of ascorbyl free radical (AFR) in a homogeneous group of 12 patients undergoing aortic valve replacement. Dimethyl sulfoxide (DMSO) was used as an enhancer and stabilizer for AFR in plasma separated from blood samples collected 15 min before incision, 10 min before aortic declamping, and sequentially during the initial 30 min of reperfusion. Plasma DMSO/AFR levels of patients were found to be significantly lower than in healthy subjects (-25%), further decreased upon ischemia (-35%), dropped to their lowest values within the first 10 min of reperfusion (-46%), and did not recover their initial values within 30 min following reflow. Cardiac index measurements revealed a still depressed heart function 4 h postdeclamping and a more delayed tissue injury was evidenced by cardiac myosin and myoglobin release in plasma. DMSO/AFR levels at early reperfusion were slightly (+ 12%) higher in plasma obtained from coronary sinus samples than in plasma from peripheral blood, suggesting an extra ascorbate release from the injured heart tissue. The close analogy between these results and the reported measurements of other plasma markers of oxidative stress, including ascorbate, indicates that the present method could be of great value in clinical practice.
为评估心脏缺血/再灌注过程中氧化应激的发展情况，通过电子自旋共振光谱检测12例接受主动脉瓣置换术患者的同质组中抗坏血酸自由基（AFR），监测血浆抗坏血酸的消耗情况。在切开前15分钟、主动脉阻断前10分钟以及再灌注初始30分钟期间依次采集血样，分离血浆，使用二甲基亚砜（DMSO）作为血浆中AFR的增强剂和稳定剂。发现患者的血浆DMSO/AFR水平显著低于健康受试者（-25%），缺血时进一步降低（-35%），在再灌注的前10分钟内降至最低值（-46%），且在再灌注后30分钟内未恢复至初始值。心脏指数测量显示，主动脉阻断后4小时心脏功能仍受抑制，血浆中心肌肌球蛋白和肌红蛋白的释放证明组织损伤出现得更晚。早期再灌注时，从冠状窦样本获得的血浆中DMSO/AFR水平比外周血血浆略高（+12%），表明受损心脏组织额外释放了抗坏血酸。这些结果与报道的其他氧化应激血浆标志物（包括抗坏血酸）的测量结果之间的密切相似性表明，本方法在临床实践中可能具有重要价值。
