Delmas-Beauvieux M C, Peuchant E, Thomas M J, Dubourg L, Pinto A P, Clerc M, Gin H
RMSB UMR 5536, CNRS-Université Victor Segalen, Bordeaux, France.
Clin Biochem. 1998 Jun;31(4):221-8. doi: 10.1016/s0009-9120(98)00019-8.
Chronic production of reactive oxygen species (ROS) and/or deficiency in the antioxidant defense system are observed in non-insulin-dependent diabetes mellitus (NIDDM) patients. As an adjunct to the usual indirect parameters for evaluating oxidative stress, we assessed the feasibility of oxyradicals detection in venous blood by electron spin resonance spectroscopy (ESR). Detection of the ascorbate pool was also performed using the validated ESR analysis of the ascorbyl free radial (AFR)-dimethyl sulfoxide (DMSO) complex.
Plasma lipoperoxidation was characterized by higher levels of total MDA (1.50 +/- 0.08 nmol/L), lower levels of GSH (0.54 +/- 0.02 mmol/L) and of vitamin A (2.13 +/- 0.52 mumol/L) in the NIDDM group than in the controls (0.75 +/- 0.05 nmol/L, 0.90 +/- 0.05 mmol/L, 3.52 +/- 1.04 mumol/L, respectively). Improvement of the ESR measurement of oxyradical adducts has been previously obtained by addition of a new sensitive nitrone (DEPMPO), which acts as a spin-trap. However, in our experiment the ESR signal-to-noise ratio was too low to detect significative oxyradicals adducts in total venous blood of NIDDM patients having a weak production of ROS. A significant difference (p < 0.002) was observed in DMSO/AFR index between controls (24.00 +/- 4.10 nmol/L) and NIDDM patients (7.28 +/- 2.36 nmol/L) suggesting ascorbate depletion related to the free radical production.
The DMSO/AFR index could be an interesting additional marker of oxidative stress during a chronic production of ROS.
在非胰岛素依赖型糖尿病（NIDDM）患者中观察到活性氧（ROS）的慢性产生和/或抗氧化防御系统的缺陷。作为评估氧化应激的常用间接参数的辅助手段，我们评估了通过电子自旋共振光谱（ESR）检测静脉血中氧自由基的可行性。还使用经过验证的抗坏血酸自由基（AFR）-二甲基亚砜（DMSO）复合物的ESR分析进行了抗坏血酸池的检测。
NIDDM组血浆脂质过氧化的特征是总丙二醛（MDA）水平较高（1.50±0.08 nmol/L），谷胱甘肽（GSH）水平较低（0.54±0.02 mmol/L），维生素A水平较低（2.13±0.52 μmol/L），而对照组分别为（0.75±0.05 nmol/L，0.90±0.05 mmol/L，3.52±1.04 μmol/L）。先前通过添加一种新的敏感硝酮（DEPMPO）（作为自旋捕获剂）改进了ESR对氧自由基加合物的测量。然而，在我们的实验中，ESR信噪比太低，无法在ROS产生较弱的NIDDM患者的全静脉血中检测到有意义的氧自由基加合物。在对照组（24.00±4.10 nmol/L）和NIDDM患者（7.28±2.36 nmol/L）之间观察到DMSO/AFR指数有显著差异（p<0.002），表明抗坏血酸消耗与自由基产生有关。
DMSO/AFR指数可能是ROS慢性产生期间氧化应激的一个有趣的附加标志物。
