Suzuki Y, Yang Q, Conley F K, Abrams J S, Remington J S
Department of Immunology and Infectious Diseases, Palo Alto Medical Foundation, California 94301.
Infect Immun. 1994 Jul;62(7):2773-8. doi: 10.1128/iai.62.7.2773-2778.1994.
Treatment of toxoplasmic encephalitis in C57BL/6 mice with monoclonal antibody (MAb) against interleukin-6 (IL-6) resulted in a remarkable decrease in the number of foci of acute inflammation in their brains caused by proliferation of tachyzoites. In brains of mice treated with isotype control MAb and those treated with anti-IL-6 MAb, tachyzoites were observed only in foci of acute inflammation. Immunoperoxidase staining revealed a greatly diminished frequency of tachyzoites in brains of mice treated with anti-IL-6 MAb. Of interest, treatment with MAb against IL-6 was also associated with reduced numbers of Toxoplasma gondii cysts in the brains and with higher serum levels of gamma interferon than in control mice. Paradoxically, the mice treated with anti-IL-6 MAb had higher serum levels of IL-6 as measured by an enzyme-linked immunosorbent assay than controls. These results revealed the importance of IL-6 in the immunopathogenesis of murine toxoplasmic encephalitis.
用抗白细胞介素-6（IL-6）单克隆抗体（MAb）治疗C57BL/6小鼠的弓形虫性脑炎，可使由速殖子增殖引起的脑部急性炎症病灶数量显著减少。在用同型对照单克隆抗体处理的小鼠大脑和用抗IL-6单克隆抗体处理的小鼠大脑中，仅在急性炎症病灶中观察到速殖子。免疫过氧化物酶染色显示，在用抗IL-6单克隆抗体处理的小鼠大脑中，速殖子的频率大大降低。有趣的是，用抗IL-6单克隆抗体治疗还与大脑中弓形虫囊肿数量减少以及血清γ干扰素水平高于对照小鼠有关。矛盾的是，通过酶联免疫吸附测定法测量，用抗IL-6单克隆抗体处理的小鼠血清IL-6水平高于对照小鼠。这些结果揭示了IL-6在小鼠弓形虫性脑炎免疫发病机制中的重要性。
