Neu T R, Verkerke G J, Herrmann I F, Schutte H K, Van der Mei H C, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Appl Bacteriol. 1994 May;76(5):521-8. doi: 10.1111/j.1365-2672.1994.tb01111.x.
Silicone rubber voice prostheses are implants which are inserted in a non-sterile environment and therefore become quickly colonized by micro-organisms. The micro-organisms exist on the medical grade silicone rubber as mixed biofilms of bacteria and yeasts. A total of 79 bacterial and 39 yeast strains were isolated from these biofilms by soft ultrasonic treatment. Gram-positive/catalase-negative and Gram-positive/catalase-positive cocci represented the dominant bacterial strains. The yeasts were mainly Candida species. Further characterization of cell surface properties such as hydrophobicity by microbial adhesion to hexadecane and electrophoretic mobility showed a distinct difference when the bacterial strains were compared with the yeasts. The bacterial hydrophobicities ranged from 0 to 100% adhesion to hexadecane, whereas the yeast strains, especially the Candida albicans strains, all had markedly hydrophilic cell surfaces. A comparison of the electrophoretic mobilities showed also differences between bacteria and yeast. The values for the bacteria were found to be between -2.5 to -0.5 (10(-8) m2 V-1 s-1), whereas for the yeasts electrophoretic mobilities were more positive. Based on the adhesive properties of the isolated micro-organisms, strategies can now be developed to modify the properties of the silicone rubber to reduce biofilm formation on such prostheses.
硅橡胶语音假体是植入物，在非无菌环境中插入，因此很快就会被微生物定植。微生物以细菌和酵母的混合生物膜形式存在于医用级硅橡胶上。通过软超声处理从这些生物膜中总共分离出79株细菌和39株酵母菌株。革兰氏阳性/过氧化氢酶阴性和革兰氏阳性/过氧化氢酶阳性球菌是主要的细菌菌株。酵母主要是念珠菌属。通过微生物对十六烷的粘附和电泳迁移率等细胞表面特性的进一步表征表明，当将细菌菌株与酵母进行比较时存在明显差异。细菌对十六烷的疏水性范围为0至100%的粘附，而酵母菌株，尤其是白色念珠菌菌株，都具有明显亲水性的细胞表面。电泳迁移率的比较也显示出细菌和酵母之间的差异。发现细菌的值在-2.5至-0.5（10^(-8) m2 V^(-1) s^(-1)）之间，而酵母的电泳迁移率更正。基于分离出的微生物的粘附特性，现在可以制定策略来改变硅橡胶的特性，以减少此类假体上生物膜的形成。
