Tsikopoulos Alexios, Tsikopoulos Konstantinos, Meroni Gabriele, Gravalidis Christoforos, Soukouroglou Prodromos, Chatzimoschou Athanasios, Drago Lorenzo, Triaridis Stefanos, Papaioannidou Paraskevi
1st Department of Pharmacology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
One Health Unit, Department of Biomedical, Surgical and Dental Sciences, School of Medicine, University of Milan, 20133 Milan, Italy.
Antibiotics (Basel). 2023 Jun 25;12(7):1103. doi: 10.3390/antibiotics12071103.
Early failure of silicone voice prostheses resulting from fungal colonization and biofilm formation poses a major concern in modern ear nose throat surgery. Therefore, developing new infection prevention techniques to prolong those implants' survivorship is crucial. We designed an in vitro laboratory study to include nanomaterial-enhanced polymer coating with a plasma spraying technique against growth to address this issue. The anti-biofilm effects of high- and low-dose AlO nanowire and TiO nanoparticle coatings were studied either alone or in conjunction with each other using checkerboard testing. It was demonstrated that both nanomaterials were capable of preventing fungal biofilm formation regardless of the anti-fungal agent concentration (median absorbance for high-dose AlO-enhanced polymer coating was 0.176 [IQR = 0.207] versus control absorbance of 0.805 [IQR = 0.381], = 0.003 [98% biofilm reduction]; median absorbance for high-dose TiO-enhanced polymer coating was 0.186 [IQR = 0.024] versus control absorbance of 0.766 [IQR = 0.458], < 0.001 [93% biofilm reduction]). Furthermore, synergy was revealed when the Bliss model was applied. According to the findings of this work, it seems that simultaneous consideration of AlO and TiO could further increase the existing antibiofilm potential of these nanomaterials and decrease the likelihood of localized toxicity.
在现代耳鼻喉科手术中，硅胶人工喉因真菌定植和生物膜形成导致的早期失效是一个主要问题。因此，开发新的感染预防技术以延长这些植入物的使用寿命至关重要。我们设计了一项体外实验室研究，采用等离子喷涂技术对纳米材料增强聚合物涂层进行抗生长研究，以解决这一问题。使用棋盘格试验单独或联合研究了高剂量和低剂量的AlO纳米线和TiO纳米颗粒涂层的抗生物膜效果。结果表明，无论抗真菌剂浓度如何，两种纳米材料都能够防止真菌生物膜形成（高剂量AlO增强聚合物涂层的中位吸光度为0.176[IQR = 0.207]，而对照吸光度为0.805[IQR = 0.381]，P = 0.003[生物膜减少98%]；高剂量TiO增强聚合物涂层的中位吸光度为0.186[IQR = 0.024]，而对照吸光度为0.766[IQR = 0.458]，P < 0.001[生物膜减少93%]）。此外，应用布利斯模型时发现了协同作用。根据这项工作的结果，似乎同时考虑AlO和TiO可以进一步提高这些纳米材料现有的抗生物膜潜力，并降低局部毒性的可能性。
