用于分离苯丁酸氮芥及其N-氧化物前药的高效液相色谱法。

High-performance liquid chromatographic method for the separation of chlorambucil and its N-oxide prodrug.

作者信息

Chandler K J, McCabe J B, Kirkpatrick D L

机构信息

Department of Chemistry, University of Regina, Sask, Canada.

出版信息

J Chromatogr. 1994 Feb 11;652(2):195-202. doi: 10.1016/0378-4347(93)e0389-8.

DOI:10.1016/0378-4347(93)e0389-8

PMID:8006104

Abstract

A reversed-phase high-performance liquid chromatographic method is described to distinguish chlorambucil N-oxide from the parent chlorambucil and quantitate both after separation from biological samples. The influence of solvent pH, alcohol, acid and ion-pairing agent on the separation is described. The stability of chlorambucil and its N-oxide in buffers and alcohols, as well as stability during filtration is discussed with potential application for metabolic studies.

摘要

描述了一种反相高效液相色谱法，用于区分苯丁酸氮芥N-氧化物与其母体苯丁酸氮芥，并在从生物样品中分离后对两者进行定量。描述了溶剂pH值、醇、酸和离子对试剂对分离的影响。讨论了苯丁酸氮芥及其N-氧化物在缓冲液和醇中的稳定性，以及过滤过程中的稳定性，并探讨了其在代谢研究中的潜在应用。