Izquierdo M, Bowden S, Cantrell D
Lymphocyte Activation Laboratory, Imperial Cancer Research Fund, London, UK.
J Exp Med. 1994 Jul 1;180(1):401-6. doi: 10.1084/jem.180.1.401.
Triggering of the T cell antigen receptor (TCR) complex activates the serine/threonine kinase Raf-1 whose function is necessary for TCR induction of the interleukin 2 gene. Raf-1 has been identified as a candidate mitogen-activated protein (MAP) kinase kinase kinase (MKKK) and thus has the potential to couple the TCR to the activation of the MAP kinases such as ERK2. In the present study, the role of Raf-1 in ERK2 regulation of ERK2 in T cells has been explored. A constitutively active Raf-1 kinase, v-raf, or a dominant inhibitory Raf-1 mutant were expressed transiently from the pEF BOS vector in Jurkat cells and the effects of these Raf-1 mutants on a coexpressed ERK2 reporter was assessed. The action of the constitutively active Raf-1 was to stimulate the ERK2 kinase, whereas the dominant negative version of Raf-1 inhibited the ERK2 activation induced by triggering of the TCR. These data indicate a role for Raf-1 in the regulation of ERK2 in T cells.
T细胞抗原受体（TCR）复合物的激活会活化丝氨酸/苏氨酸激酶Raf-1，其功能对于TCR诱导白细胞介素2基因是必需的。Raf-1已被鉴定为一种候选的丝裂原活化蛋白（MAP）激酶激酶激酶（MKKK），因此有潜力将TCR与诸如ERK2等MAP激酶的激活相偶联。在本研究中，已探讨了Raf-1在T细胞中对ERK2的调节作用。组成型活性Raf-1激酶v-raf或显性抑制性Raf-1突变体从pEF BOS载体在Jurkat细胞中瞬时表达，并评估了这些Raf-1突变体对共表达的ERK2报告基因的影响。组成型活性Raf-1的作用是刺激ERK2激酶，而Raf-1的显性负性形式则抑制由TCR触发诱导的ERK2激活。这些数据表明Raf-1在T细胞中ERK2的调节中起作用。
