Labropoulou V, Balamotis A, Tosca A, Rotola A, Mavromara-Nazos P
Department of Virology, Hellenic Pasteur Institute, Athens, Greece.
J Med Virol. 1994 Mar;42(3):259-63. doi: 10.1002/jmv.1890420311.
DNA samples from recurrent condylomata acuminata biopsies of Greek males and females were examined for the presence of human papillomavirus (HPV) DNA using high-stringency Southern blot hybridization analysis. Of the twenty-six biopsies, 25 were positive for the HPV 6/11-related DNA sequences, and when further analyzed with the polymerase chain reaction (PCR) the HPV-negative biopsy was also positive for HPV 6/11 DNA. Nineteen specimens were further characterized based on their Pstl restriction endonuclease hybridization pattern. Twelve biopsies were positive for HPV 6a, one biopsy was positive for HPV 11a, and one biopsy was positive for HPV 6c DNA. Three specimens contained HPV 6/11 related DNA that gave an unusual Pstl pattern, and one specimen appeared to represent a multiple HPV infection containing HPV 6/11- and HPV 31/35/39-related sequences. Finally, one sample contained a mixture of HPV 6a DNA and an HPV 6a-like genome. Biopsies were also taken from adjacent apparently normal tissue, 0.5 cm away from the lesion, in 19 of the patients. Only one of these was found to be positive for HPV 6a DNA by Southern blot analysis.
采用高严谨度Southern印迹杂交分析法，对希腊男性和女性复发性尖锐湿疣活检组织的DNA样本进行人乳头瘤病毒（HPV）DNA检测。在26份活检组织中，25份HPV 6/11相关DNA序列呈阳性，当采用聚合酶链反应（PCR）进一步分析时，HPV阴性的活检组织HPV 6/11 DNA也呈阳性。根据Pstl限制性内切酶杂交图谱，对19份标本进行了进一步鉴定。12份活检组织HPV 6a呈阳性，1份活检组织HPV 11a呈阳性，1份活检组织HPV 6c DNA呈阳性。3份标本含有HPV 6/11相关DNA，其Pstl图谱异常，1份标本似乎代表多重HPV感染，含有HPV 6/11和HPV 31/35/39相关序列。最后，1份样本含有HPV 6a DNA和类HPV 6a基因组的混合物。对19例患者，还从距病变0.5 cm的相邻明显正常组织取材进行活检。经Southern印迹分析，其中仅1份HPV 6a DNA呈阳性。
