Liu Y, Wang J, Si J
PUMC Hospital, CAMS & PUMC, Beijing.
Chin Med Sci J. 1996 Mar;11(1):53-5.
This study differentiated pseudocondyloma of vulva from condyloma acuminata using dot blot hybridization and polymerase chain reaction (PCR). A total of 27 cases of pseudocondyloma of vulva and 65 cases of condyloma acuminata were selected for the study. The genital lesions were examined clinically and were biopsied. Each biopsy was subjected to histological examination and HPV DNA analysis by dot blot hybridization and PCR. Dot blot analysis detected HPV DNA in 19(82.6%) out of 23 cases of condyloma acuminata and 2(25%) out of 8 cases pseudocondyloma of vulvae (P < 0.05). PCR detected HPV DNA in 51(92.7%) out of 55 cases of condyloma acuminata, compared with none in 23 cases of pseudocondyloma (P < 0.001). HPV DNA was present in the majority of condyloma acuminata specimens, HPV 6 and 11 were the predominant types. Peudocondyloma is probably not associated with HPV. PCR was the most sensitive and useful technique for HPV DNA detection.
本研究采用斑点杂交和聚合酶链反应（PCR）对外阴假性湿疣和尖锐湿疣进行鉴别。共选取27例外阴假性湿疣患者和65例尖锐湿疣患者作为研究对象。对生殖器病变进行临床检查并取活检。每例活检标本均进行组织学检查以及通过斑点杂交和PCR进行人乳头瘤病毒（HPV）DNA分析。斑点杂交分析在23例尖锐湿疣中有19例（82.6%）检测到HPV DNA，而在8例外阴假性湿疣中有2例（25%）检测到HPV DNA（P<0.05）。PCR在55例尖锐湿疣中有51例（92.7%）检测到HPV DNA，相比之下，23例假性湿疣中无一例检测到（P<0.001）。大多数尖锐湿疣标本中存在HPV DNA，HPV 6和11为主要类型。假性湿疣可能与HPV无关。PCR是检测HPV DNA最敏感且有用的技术。
