Mitchell M D, Edwin S S, Lundin-Schiller S, Silver R M, Smotkin D, Trautman M S
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Placenta. 1993 Nov-Dec;14(6):615-25. doi: 10.1016/s0143-4004(05)80379-0.
We have evaluated the mechanism by which interleukin-1 beta (IL-1 beta) increases amnion cell PGE2 production in a concentration-dependent manner. IL-1 beta-stimulated amnion cell PGE2 biosynthesis was time-dependent, and significant stimulation occurred within 2 h of incubation. IL-1 beta stimulation occurred in the presence of added arachidonic acid but was abrogated by treatment with cycloheximide and actinomycin D. Amnion cells treated with IL-1 beta recovered rapidly from aspirin pretreatment suggesting an action on fatty acid cyclooxygenase (COX). Increased amounts of COX protein were demonstrated by Western blot analysis within 2 h of IL-1 beta treatment of amnion cells. Northern blot analysis using a probe specific for a novel form of COX (COX-II) showed an increase in mRNA for this COX within 30 min. This finding using a homologous detection system and human cells of fetal origin in primary culture provides strong support for a physiological role for COX-II in man.
我们已经评估了白细胞介素-1β（IL-1β）以浓度依赖方式增加羊膜细胞前列腺素E2（PGE2）产生的机制。IL-1β刺激的羊膜细胞PGE2生物合成是时间依赖性的，在孵育2小时内出现显著刺激。IL-1β刺激在添加花生四烯酸的情况下发生，但用环己酰亚胺和放线菌素D处理可消除这种刺激。用IL-1β处理的羊膜细胞从阿司匹林预处理中迅速恢复，提示其对脂肪酸环氧化酶（COX）有作用。通过蛋白质印迹分析表明，在IL-1β处理羊膜细胞2小时内COX蛋白量增加。使用针对新型COX（COX-II）的特异性探针进行的Northern印迹分析显示，在30分钟内该COX的mRNA增加。在原代培养中使用同源检测系统和源自胎儿的人类细胞的这一发现为COX-II在人类中的生理作用提供了有力支持。
