Nitta K, Uchida K
Department of Medicine, Tokyo Women's Medical College.
Nihon Rinsho. 1994 May;52(5):1389-95.
Glomerular endothelial cells (GEN) have recently been cloned, cultivated and characterized. They have as same biological characteristics as expressed in endothelial cells derived from different sources. Because of the difficulties of cloning of GEN, we first transfected plasmid DNA containing large T antigen of SV-40 (NH-GEN1). Culture method of GEN and NH-GEN1 and its application are discussed.
肾小球内皮细胞(GEN)最近已被克隆、培养和鉴定。它们具有与源自不同来源的内皮细胞所表达的相同生物学特性。由于克隆GEN存在困难,我们首先转染了含有SV - 40大T抗原的质粒DNA(NH - GEN1)。本文讨论了GEN和NH - GEN1的培养方法及其应用。