Tomoda K, Yoneda T, Tsukaguchi K, Yoshikawa M, Tokuyama T, Fu A, Fukuoka K, Nakaya M, Narita N, Tasaka H
Second Department of Internal Medicine of Nara Medical University, Japan.
Kekkaku. 1994 May;69(5):361-5.
We investigated the responsiveness of peripheral blood lymphocytes (PBLs) from patients with M. avium-intracellulare complex (MAC) infection to the stimulation with PPDs by measuring their IFN-gamma producing ability. PBLs were obtained from MAC patients at active stage (culture-positive after three-month chemotherapy), those at inactive stage (culture-negative for three months after or during chemotherapy), and healthy donors. PPDs used were PPD-S prepared from M. tuberculosis, PPD-B from M. intracellulare, and PPD-Y from M. kansasii. PBLs from active MAC patients did not produce IFN-gamma to a significant extent by stimulation with any of three PPDs, while PBLs from inactive MAC patients showed higher responses to each PPD compared to those from active patients. In inactive MAC patients, the maximal response was observed to PPD-B among three PPDs. On the other hand, PBLs from healthy controls produced different levels of IFN-gamma in response to three different PPDs, and their response was most remarkable to PPD-S. These results indicated that the responsiveness of patients' PBLs to PPDs was impaired during active stage of MAC infection and restored on recovery from the disease.
我们通过测量外周血淋巴细胞（PBLs）产生γ干扰素的能力，研究了鸟分枝杆菌胞内复合群（MAC）感染患者的PBLs对结核菌纯蛋白衍生物（PPDs）刺激的反应性。PBLs取自MAC感染活跃期患者（化疗三个月后培养呈阳性）、非活跃期患者（化疗后或化疗期间三个月培养呈阴性）以及健康供者。所用的PPDs分别是由结核分枝杆菌制备的PPD-S、胞内分枝杆菌的PPD-B和堪萨斯分枝杆菌的PPD-Y。活跃期MAC患者的PBLs经三种PPDs中的任何一种刺激后，均未显著产生γ干扰素，而非活跃期MAC患者的PBLs对每种PPD的反应均高于活跃期患者。在非活跃期MAC患者中，三种PPDs中对PPD-B的反应最大。另一方面，健康对照者的PBLs对三种不同PPDs的反应产生不同水平的γ干扰素，且对PPD-S的反应最为显著。这些结果表明，患者的PBLs在MAC感染活跃期对PPDs的反应性受损，疾病康复后恢复。
