Coons S W, Johnson P C, Pearl D K, Olafsen A G
Division of Neuropathology, Barrow Neurological Institute, Phoenix, Arizona.
Neurosurgery. 1994 Apr;34(4):680-7; discussion 687.
Flow cytometry was used to determine the deoxyribonucleic acid ploidy and proliferative activity of 60 oligodendrogliomas and oligoastrocytomas. The relationships among survival, ploidy, proliferation, histological features, and clinical variables were analyzed. Survival was strongly associated with the S-phase fraction (P < 0.001). Three groups with significantly different survival rates were defined, based on S-phase fraction ranges of < 3%, 3 to 5.9%, and > 6%. Significant associations between survival and age at diagnosis (P < 0.001), tumor grade (P < 0.001), and extent of surgery (P < 0.01) were found also. The poor correlation between mitotic figures and survival in oligodendrogliomas that has been reported previously emphasizes the need for an accurate method to measure proliferative activity. Our study demonstrated the usefulness of the flow cytometry-determined S-phase fraction in this regard and demonstrated that specific reference ranges could be defined for clinical application. In contrast, the determination of ploidy by flow cytometry was not useful in the evaluation of oligodendrogliomas.
采用流式细胞术检测60例少突胶质细胞瘤和少突星形细胞瘤的脱氧核糖核酸倍体及增殖活性。分析了生存、倍体、增殖、组织学特征和临床变量之间的关系。生存与S期分数密切相关（P<0.001）。根据S期分数范围<3%、3%至5.9%和>6%，定义了三组生存率有显著差异的患者。还发现生存与诊断时年龄（P<0.001）、肿瘤分级（P<0.001）和手术范围（P<0.01）之间存在显著关联。先前报道的少突胶质细胞瘤有丝分裂指数与生存之间的低相关性强调了需要一种准确的方法来测量增殖活性。我们的研究证明了流式细胞术测定的S期分数在这方面的有用性，并证明可以为临床应用定义特定的参考范围。相比之下，通过流式细胞术测定倍体在少突胶质细胞瘤的评估中并无用处。
