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[通过形态学研究方法组合显示早期局部生命反应(作者译)]

[Demonstration of early local vital reactions by combination of morphological investigation methods (author's transl)].

作者信息

Böhm E

出版信息

Z Rechtsmed. 1978 Jul 27;81(3):191-206. doi: 10.1007/BF00201268.

Abstract

In extension of earlier Scanning Electron Microscopical findings on local vital reaction, we initially tried conventional microscopic histology. By standard staining methods--generally used in demonstrating fibrins (Ladew IG, PTAH, PAS etc.) fresh fibrinous mashing was hardly demonstrable. Tryptophan reaction as per Adams brought positive results. By modifying Scanning Electron Microscopic preparation techniques, the surface transverse striation could be shown. For dissolution of the complex morphological structural patterns on the wound surface, Transmission Electron Microscopy was used. Identification of fibrinous filaments was done by their interior transverse striation. Mashing of shaped structure components of the blood in vital and post mortal injuries should be more closely analyzed. Advantages and disadvantages of Scanning and Transmission Electron Microscopic techniques are discussed in view of forensic problems and a tentative judgement passed.

摘要

在早期关于局部生命反应的扫描电子显微镜研究结果的基础上,我们首先尝试了传统的显微组织学方法。通过通常用于显示纤维蛋白的标准染色方法(如Ladew IG、PTAH、PAS等),新鲜的纤维蛋白捣碎物几乎无法显示。按照亚当斯的方法进行的色氨酸反应得到了阳性结果。通过改进扫描电子显微镜制备技术,可以显示表面横向条纹。为了解伤口表面复杂的形态结构模式,使用了透射电子显微镜。通过纤维丝内部的横向条纹来识别纤维蛋白丝。对于生前和死后损伤中血液成形结构成分的捣碎情况,应进行更深入的分析。鉴于法医问题,讨论了扫描电子显微镜和透射电子显微镜技术的优缺点,并做出了初步判断。

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