Totsune K, Takahashi K, Murakami O, Itoi K, Sone M, Ohneda M, Satoh F, Miura Y, Mouri T
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Peptides. 1994;15(2):287-90. doi: 10.1016/0196-9781(94)90014-0.
C-type natriuretic peptide (CNP) in human adrenal glands and adrenal tumors was measured with a specific radioimmunoassay for CNP. Tissue immunoreactive (IR-) CNP concentrations were 0.54 +/- 0.40 pmol/g wet tissue (gwt) (mean +/- SD) in 14 pheochromocytomas, 0.69 +/- 0.19 pmol/gwt in six adrenocortical tumors, and 0.49 +/- 0.22 pmol/gwt in seven normal adrenal glands (cortex and medulla mixed). These concentrations were comparable to those found in tissues from human brains. Sephadex G-50 superfine column chromatography and reverse-phase high performance liquid chromatography revealed that IR-CNP in normal adrenal glands and pheochromocytoma consisted of at least two components: a component in low molecular weight form chromatographically identical to CNP-22 and the other, a high molecular weight form very similar to human CNP-53. This study has shown that IR-CNP is present in human adrenal glands and adrenal tumors with similar molecular forms and comparable concentrations to those in the human brain.
采用针对C型利钠肽（CNP）的特异性放射免疫分析法，测定人肾上腺及肾上腺肿瘤中的CNP。14例嗜铬细胞瘤组织免疫反应性（IR-）CNP浓度为0.54±0.40 pmol/g湿组织（gwt）（均值±标准差），6例肾上腺皮质肿瘤中为0.69±0.19 pmol/gwt，7例正常肾上腺（皮质和髓质混合）中为0.49±0.22 pmol/gwt。这些浓度与在人脑组织中发现的浓度相当。葡聚糖G-50超细柱色谱法和反相高效液相色谱法显示，正常肾上腺和嗜铬细胞瘤中的IR-CNP至少由两种成分组成：一种低分子量形式的成分，其色谱行为与CNP-22相同，另一种高分子量形式的成分与人类CNP-53非常相似。本研究表明，IR-CNP存在于人肾上腺及肾上腺肿瘤中，其分子形式和浓度与在人脑中的相似。
