Satoh F, Takahashi K, Murakami O, Totsune K, Sone M, Ohneda M, Abe K, Miura Y, Mouri T
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Cancer Detect Prev. 1997;21(1):51-4.
Adrenomedullin is a potent vasodilator peptide that was isolated from human pheochromocytoma. We developed a sensitive and specific radioimmunoassay for adrenomedullin and studied the presence of adrenomedullin in human adrenal glands and adrenal tumors, including pheochromocytoma. High concentrations of immunoreactive adrenomedullin were found in normal parts of adrenal glands (cortex and medulla) (12.6 +/- 1.0 pmol/g wet wt, N = 7, mean +/- SEM). High concentrations of immunoreactive adrenomedullin were also present in the tumor tissues of pheochromocytoma (4.5 +/- 1.5 pmol/g wet wt, N = 11). Immunoreactive adrenomedullin was detected in some adrenocortical tumors, but these concentrations were much lower than those in the normal adrenal glands and pheochromocytomas. Reverse phase high-performance liquid chromatography of the normal adrenal gland and pheochromocytoma showed a peak eluting in the position of synthetic adrenomedullin 1-52. The present study has shown the presence of high concentrations of immunoreactive adrenomedullin in the normal adrenal glands and pheochromocytomas.
肾上腺髓质素是一种从人嗜铬细胞瘤中分离出的强效血管舒张肽。我们开发了一种灵敏且特异的肾上腺髓质素放射免疫测定法，并研究了肾上腺髓质素在人肾上腺及肾上腺肿瘤（包括嗜铬细胞瘤）中的存在情况。在肾上腺的正常部分（皮质和髓质）发现了高浓度的免疫反应性肾上腺髓质素（12.6±1.0 pmol/g湿重，N = 7，平均值±标准误）。嗜铬细胞瘤的肿瘤组织中也存在高浓度的免疫反应性肾上腺髓质素（4.5±1.5 pmol/g湿重，N = 11）。在一些肾上腺皮质肿瘤中检测到了免疫反应性肾上腺髓质素，但这些浓度远低于正常肾上腺和嗜铬细胞瘤中的浓度。正常肾上腺和嗜铬细胞瘤的反相高效液相色谱显示，在合成肾上腺髓质素1 - 52的位置出现一个洗脱峰。本研究表明，正常肾上腺和嗜铬细胞瘤中存在高浓度的免疫反应性肾上腺髓质素。
