Lanza G, Maestri I, Ballotta M R, Dubini A, Cavazzini L
Istituto di Anatomia, Istologia e Citologia Patologica, Università di Ferrara, Italy.
Mod Pathol. 1994 Feb;7(2):161-5.
Nuclear DNA content was determined in 123 colorectal adenocarcinomas by flow cytometry using multiple frozen tumor samples. Thirty-three (26.8%) carcinomas were classified as diploid and 90 as aneuploid (73.2%). Presence of DNA aneuploidy was found to be unrelated to tumor stage and grade of differentiation and to other histopathological variables such as pattern of growth, degree of peritumoral lymphocytic infiltration, and venous invasion. However, multiploid tumors (20/123, 16.3%) were more frequently noted in advanced stages of disease (Stages III and IV, P < 0.025) and more often showed unfavorable histopathological features, especially an infiltrating pattern of growth (P < 0.05), compared with diploid and single aneuploid carcinomas. Nuclear DNA content was found to be closely related to tumor site. Carcinomas of the proximal (right and transverse) colon were more frequently diploid (19/43, 44.2% versus 14/80, 17.5%--P < 0.005) and more often displayed a DNA index (DI, defined as the ratio of the DNA content of neoplastic cells to that of normal cells) < or = 1.20 (27/43, 62.8% versus 19/80, 23.7%--P < 0.001) than did tumors localized distally to the splenic flexure. Nuclear DNA content was also found to be related to tumor type. A high proportion of mucinous adenocarcinomas showed DI values < or = 1.20 (14/21, 66.7%); conversely only 32 of 102 (31.4%) non-mucinous adenocarcinomas had a DI < or = 1.20 (P < 0.01). The nuclear DNA content of mucinous adenocarcinomas seemed to be independent of tumor location.(ABSTRACT TRUNCATED AT 250 WORDS)
采用多个冷冻肿瘤样本，通过流式细胞术测定了123例大肠腺癌的核DNA含量。33例（26.8%）癌被分类为二倍体，90例为非整倍体（73.2%）。发现DNA非整倍体的存在与肿瘤分期、分化程度以及其他组织病理学变量如生长模式、肿瘤周围淋巴细胞浸润程度和静脉侵犯无关。然而，与二倍体和单倍体非整倍体癌相比，多倍体肿瘤（20/123，16.3%）在疾病晚期（III期和IV期，P<0.025）更常见，且更常表现出不良的组织病理学特征，尤其是浸润性生长模式（P<0.05）。发现核DNA含量与肿瘤部位密切相关。近端（右半结肠和横结肠）结肠癌更常为二倍体（19/43，44.2%，而14/80，17.5%，P<0.005），且与脾曲远端的肿瘤相比，更常表现出DNA指数（DI，定义为肿瘤细胞与正常细胞DNA含量之比）≤1.20（27/43，62.8%，而19/80，23.7%，P<0.001）。还发现核DNA含量与肿瘤类型有关。高比例的黏液腺癌显示DI值≤1.20（14/21，66.7%）；相反，102例非黏液腺癌中只有32例（31.4%）的DI≤1.20（P<0.01）。黏液腺癌的核DNA含量似乎与肿瘤位置无关。（摘要截短于250字）
