Bernardi A, Gaillard C, Bernardi G
Eur J Biochem. 1975 Apr 1;52(3):451-7. doi: 10.1111/j.1432-1033.1975.tb04013.x.
The 5'-terminal dinucleotides released by five deoxyribonucleases (spleen acid DNase, snail acid DNase, pancreatic DNase, Escherichia coli endonuclease I and crab DNase) have been determined on E. coli DNA (51% dG & dC) digests having different average sizes (Pn) in the range 50 to 10. It has been shown that the composition of the 5'-terminal dinucleotide (a) is independent upon the degradation level, at least in the range explored; (b) is strongly different from the composition of E. coli DNA doublets, these differences being characteristic for each enzyme; (c) is very significantly different from the statistical composition of 5'-terminal dinucleotides, as calculated from the composition of 5'-terminal and penultimate nucleotides. A calculation of the statistical composition of the trinucleotides split by each enzyme, using the 3'-terminal nucleotide data in conjunction with the 5'-terminal dinucleotide results provided a qualitative "specificity spectrum" for each enzyme.
已对大肠杆菌DNA（51%为dG和dC）进行消化，这些消化产物具有50至10范围内不同的平均大小（Pn），并确定了五种脱氧核糖核酸酶（脾酸脱氧核糖核酸酶、蜗牛酸脱氧核糖核酸酶、胰脱氧核糖核酸酶、大肠杆菌内切核酸酶I和蟹脱氧核糖核酸酶）释放的5'-末端二核苷酸。结果表明，5'-末端二核苷酸（a）的组成与降解水平无关，至少在所研究的范围内如此；（b）与大肠杆菌DNA双联体的组成有很大差异，这些差异对每种酶而言都是独特的；（c）与根据5'-末端和倒数第二个核苷酸的组成计算出的5'-末端二核苷酸的统计组成有非常显著的差异。利用3'-末端核苷酸数据结合5'-末端二核苷酸的结果，计算每种酶切割的三核苷酸的统计组成，为每种酶提供了一个定性的“特异性谱”。
