• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用多核苷酸激酶和核苷酸“图谱”方法进行核苷酸序列分析。来自噬菌体λ和424的脱氧核糖核酸的5'-末端序列。

Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.

作者信息

Murray K

出版信息

Biochem J. 1973 Mar;131(3):569-82. doi: 10.1042/bj1310569.

DOI:10.1042/bj1310569
PMID:4352720
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177504/
Abstract

The polynucleotide kinase reaction was used in analyses of complex mixtures of oligodeoxynucleotides which were fractionated by various two-dimensional nucleotide ;mapping' procedures. Parallel ionophoretic analyses on DEAE-cellulose paper, pH2, and AE-cellulose paper, pH3.5, of venom phosphodiesterase partial digests of 5'-terminally labelled oligonucleotides enabled the sequence of the nucleotides to be deduced uniquely. A ;diagonal ionophoresis' method has been used with mixtures of nucleotides. Application of these methods to 5'-terminally labelled DNA from bacteriophage lambda gave the terminal sequences pA-G-G-T-C-G and pG-G-G-C-G. Identical 5'-terminal sequences were found with DNA from bacteriophage 424.

摘要

多核苷酸激酶反应被用于分析通过各种二维核苷酸“图谱”程序分级分离的寡脱氧核苷酸复杂混合物。对5'-末端标记的寡核苷酸的毒液磷酸二酯酶部分消化产物在pH2的DEAE-纤维素纸和pH3.5的AE-纤维素纸上进行平行离子电泳分析，能够唯一地推断出核苷酸序列。一种“对角线离子电泳”方法已用于核苷酸混合物。将这些方法应用于来自噬菌体λ的5'-末端标记的DNA，得到末端序列pA-G-G-T-C-G和pG-G-G-C-G。在来自噬菌体424的DNA中发现了相同的5'-末端序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/dca35646aa6a/biochemj00613-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/527175c22e09/biochemj00613-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/3e71ef32ce9a/biochemj00613-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/05460bf4fcb8/biochemj00613-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/0c6da99c4a8a/biochemj00613-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/dca35646aa6a/biochemj00613-0157-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/527175c22e09/biochemj00613-0158-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/3e71ef32ce9a/biochemj00613-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/05460bf4fcb8/biochemj00613-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/0c6da99c4a8a/biochemj00613-0156-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfd7/1177504/dca35646aa6a/biochemj00613-0157-a.jpg

相似文献

1
Nucleotide sequence analysis with polynucleotide kinase and nucleotide "mapping" methods. 5'-Terminal sequences of deoxyribonucleic acid from bacteriophages lambda and 424.使用多核苷酸激酶和核苷酸“图谱”方法进行核苷酸序列分析。来自噬菌体λ和424的脱氧核糖核酸的5'-末端序列。
Biochem J. 1973 Mar;131(3):569-82. doi: 10.1042/bj1310569.
2
The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.噬菌体λDNA的3'末端核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1151-5. doi: 10.1073/pnas.70.4.1151.
3
Terminal nucleotide sequences of DNA from temperate coliphages.温和型大肠杆菌噬菌体DNA的末端核苷酸序列。
Nat New Biol. 1973 May 30;243(126):134-9. doi: 10.1038/newbio243134a0.
4
The 3'-terminal heptanucleotide sequence of the l strand of T7 deoxyribonucleic acid.T7脱氧核糖核酸l链的3'末端七核苷酸序列。
J Biol Chem. 1973 Oct 25;248(20):7001-6.
5
DNA nucleotide sequence restricted by the RI endonuclease.受RI核酸内切酶限制的DNA核苷酸序列。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448-52. doi: 10.1073/pnas.69.11.3448.
6
The 3'-terminal nucleotide sequences of T7 DNA.T7 DNA的3'末端核苷酸序列。
J Mol Biol. 1972 May 14;66(2):209-24. doi: 10.1016/0022-2836(72)90474-3.
7
Enzymatic synthesis of deoxyribonucleic acids with repeating sequences. A new repeating trinucleotide deoxyribonucleic acid, d(T-C-C)n-d(G-G-A)n.具有重复序列的脱氧核糖核酸的酶促合成。一种新的重复三核苷酸脱氧核糖核酸，d(T-C-C)n-d(G-G-A)n。
Biochemistry. 1974 Apr 9;13(8):1596-603. doi: 10.1021/bi00705a007.
8
Nucleotide clusters in in deoxyribonucleic acids. X. Sequences of the pyrimidine oligonucleotides of mouse L-cell satellite DNA.
Biochem Biophys Res Commun. 1974 Jun 4;58(3):814-21. doi: 10.1016/s0006-291x(74)80490-0.
9
A catalogue of the pyrimidine oligodeoxyribonucleotides found in bacteriophage f1 DNA.噬菌体f1 DNA中嘧啶寡脱氧核糖核苷酸的目录。
Virology. 1974 Jan;57(1):64-76. doi: 10.1016/0042-6822(74)90108-1.
10
Recognition sequence of a restriction enzyme.限制酶的识别序列。
Nat New Biol. 1973 Jul 4;244(131):7-10. doi: 10.1038/newbio244007a0.

引用本文的文献

1
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
Nucleic Acids Res. 1974 Mar;1(3):331-53. doi: 10.1093/nar/1.3.331.
2
Structure and variation of human ribosomal DNA: the external transcribed spacer and adjacent regions.人类核糖体DNA的结构与变异：外部转录间隔区及相邻区域
Am J Hum Genet. 1982 Jan;34(1):32-49.
3
The stereochemical course of phosphoryl transfer catalysed by polynucleotide kinase (bacteriophage-T4-infected Escherichia coli B).

本文引用的文献

1
The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease.大肠杆菌的脱氧核糖核酸酶。II. 一种核糖核酸抑制性内切核酸酶的纯化及特性
J Biol Chem. 1962 Mar;237:819-28.
2
The frequencies of certain sequences of nucleotides in deoxyribonucleic acid.脱氧核糖核酸中特定核苷酸序列的频率。
Biochem J. 1960 Apr;75(1):17-27. doi: 10.1042/bj0750017.
3
The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage lambda-dg.用从噬菌体λ-dg分离的脱氧核糖核酸对大肠杆菌进行转化。
多核苷酸激酶（噬菌体T4感染的大肠杆菌B）催化的磷酰基转移的立体化学过程。
Biochem J. 1981 Oct 1;199(1):273-6. doi: 10.1042/bj1990273.
4
Nucleotide sequence of the region of the origin of replication of the broad host range plasmid RK2.广宿主范围质粒RK2复制起点区域的核苷酸序列。
Mol Gen Genet. 1981;181(1):8-12. doi: 10.1007/BF00338997.
5
The nucleotide sequences recognized by endonucleases AvaI and AvaII from Anabaena variabilis.可变鱼腥藻中内切核酸酶AvaI和AvaII识别的核苷酸序列。
Biochem J. 1980 Jan 1;185(1):65-75. doi: 10.1042/bj1850065.
6
The isolation and characterization of a sequence-specific endonuclease from Anabaena subcylindrica.从圆柱鱼腥藻中分离并鉴定一种序列特异性核酸内切酶。
Biochem J. 1980 Jan 1;185(1):59-63. doi: 10.1042/bj1850059.
7
Selective protection of 5' ... GGCC ... 3' and 5' ... GCNGC ... 3' sequences by the hypermodified oxopyrimidine in Bacillus subtilis bacteriophage SP10 DNA.枯草芽孢杆菌噬菌体SP10 DNA中经超修饰的氧嘧啶对5'... GGCC... 3'和5'... GCNGC... 3'序列的选择性保护
J Virol. 1984 Oct;52(1):47-54. doi: 10.1128/JVI.52.1.47-54.1984.
8
Polynucleotides associated with histone preparations.与组蛋白制剂相关的多核苷酸。
Biochem J. 1973 Mar;131(3):585-91. doi: 10.1042/bj1310585.
9
Improved preparation of bacteriophage T4-infected Escherichia coli for the isolation of polynucleotide kinase.改进用于分离多核苷酸激酶的T4噬菌体感染的大肠杆菌的制备方法。
Biochem J. 1973 Mar;131(3):583. doi: 10.1042/bj1310583.
10
The 3'-terminal nucleotide sequences of bacteriophage lambda DNA.噬菌体λDNA的3'末端核苷酸序列。
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1151-5. doi: 10.1073/pnas.70.4.1151.
J Mol Biol. 1960 Dec;2:392-415. doi: 10.1016/s0022-2836(60)80050-2.
4
Choline phosphokinase.胆碱磷酸激酶
J Biol Chem. 1953 May;202(1):431-44.
5
The 5'-terminal nucleotides of T7 bacteriophage deoxyribonucleic acid.T7噬菌体脱氧核糖核酸的5'-末端核苷酸
J Mol Biol. 1966 Jan;15(1):49-61. doi: 10.1016/s0022-2836(66)80208-5.
6
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
7
Ter, a function which generates the ends of the mature lambda chromosome.Ter，一种生成成熟λ染色体末端的功能。
Nature. 1969 Jan 18;221(5177):242-4. doi: 10.1038/221242a0.
8
Terminal sequences of bacteriophage RNAs.噬菌体RNA的末端序列。
Nature. 1968 Nov 9;220(5167):548-52. doi: 10.1038/220548a0.
9
The stepwise removal of histones from chicken erythrocyte nucleoprotein.从鸡红细胞核蛋白中逐步去除组蛋白。
Biochem J. 1968 Mar;107(2):207-15. doi: 10.1042/bj1070207.
10
Measurement of 32P activity in a liquid scintillation counter without the use of scintillator.在不使用闪烁体的情况下，通过液体闪烁计数器测量³²P 活度。
Anal Biochem. 1968 Jan;22(1):70-3. doi: 10.1016/0003-2697(68)90260-1.