[反相高效液相色谱法测定赤霉素A3]
[Determination of gibberellin A3 by reversed phase high performance liquid chromatography].
作者信息
Zhu S D, Zhao S, Zhao W J, Zhao S H
机构信息
Department of Biochemistry, Xuzhou Medical College.
出版信息
Yao Xue Xue Bao. 1993;28(10):788-91.
The analysis of gibberellin GA3 by high performance liquid chromatography (HPLC) was carried out with mu Bondapak C18 column. The developed reversed phase HPLC procedure enables the separation of different GAs with high resolution by means of ionic suppression, including the separation of double bond isomers GA1 and GA3. This method is convenient (without the necessity of derivatization), rapid (15 min for one overall run), sensitive (0.5 microgram is sufficient for precise assay), and has fine linearity (r = 0.999, n = 5) and reproducibility (relative standard deviation < 2%, n = 4).
采用μ Bondapak C18柱通过高效液相色谱法（HPLC）对赤霉素GA3进行分析。所建立的反相高效液相色谱方法通过离子抑制能够以高分辨率分离不同的赤霉素，包括双键异构体GA1和GA3的分离。该方法简便（无需衍生化）、快速（一次全运行需15分钟）、灵敏（0.5微克足以进行精确测定），并且具有良好的线性（r = 0.999，n = 5）和重现性（相对标准偏差<2%，n = 4）。
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