Zhang Y, Ye Q X, Liu J, Zhang Z Y, Zhang T M
Department of Pharmacology, Henan Institute of Medical Sciences, Zhengzhou, China.
Zhongguo Yao Li Xue Bao. 1994 Jan;15(1):56-9.
Using 3-(4,5-dimethythiazole)-2,5-diphenyltetrazolium bromide (MTT) method, the effect of probimane (Pro) on doxorubicin (Dox) cytotoxicity was studied. Pro 0.313, 0.625, and 1.25 micrograms.ml-1 potentiated cytotoxicity of Dox in Ehrlich ascites carcinoma (EAC) cells. Incubation of EAC cells with Dox 10 micrograms.ml-1 and Pro 116.5, 233, and 466 micrograms.ml-1 resulted in an increase in intracellular drug accumulation from 0.69 +/- 0.06 to 1.08 +/- 0.10 micrograms/10(7) cells. In S37-bearing mice, Pro 23.3, 46.6, and 116.5 micrograms.ml-1 enhanced the malondialdehyde (MDA) formation in tumor and liver mitochondria and decreased MDA formation in liver mitochondria. These results suggested that the increases of Dox accumulation and MDA formation in tumor cells by Pro might be the reasons for synergistic effect of Pro on Dox cytotoxicity.
采用3-(4,5-二甲基噻唑)-2,5-二苯基四氮唑溴盐(MTT)法，研究了丙亚胺(Pro)对阿霉素(Dox)细胞毒性的影响。丙亚胺0.313、0.625和1.25微克·毫升-1增强了阿霉素对艾氏腹水癌(EAC)细胞的细胞毒性。用10微克·毫升-1阿霉素和116.5、233和466微克·毫升-1丙亚胺孵育EAC细胞，导致细胞内药物蓄积从0.69±0.06微克/10(7)细胞增加到1.08±0.10微克/10(7)细胞。在荷S37小鼠中，23.3、46.6和116.5微克·毫升-1丙亚胺增强了肿瘤和肝脏线粒体中丙二醛(MDA)的形成，降低了肝脏线粒体中MDA的形成。这些结果表明，丙亚胺增加肿瘤细胞中阿霉素的蓄积和MDA的形成可能是丙亚胺对阿霉素细胞毒性产生协同作用的原因。
