汉防己甲素联合阿霉素或长春新碱体外协同抗癌作用
Synergistic anticancer effects of tetrandrine combined with doxorubicin or vincristine in vitro.
作者信息
Sun A X, Ye Z G, Li C Y, Xue B Y, Li L F, Cao X F, Yang Q, Dai B Q
机构信息
Department of Pharmacology, Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, China.
出版信息
Zhongguo Yao Li Xue Bao. 1999 Jan;20(1):69-73.
AIM
To study the interaction between tetrandrine (Tet) and doxorubicin (Dox) or vincristine (Vin) against human breast cancer cell lines MCF-7 and MCF-7/Dox or human nasopharyngeal cancer cell lines KB and KBV200 in vitro.
METHODS
Anticancer activities of a drug alone and a drug combination of Tet and Dox or Vin were determined by tetrazolium (MTT) method. The interaction between Tet and Dox or Vin was evaluated by both a value of a sum of fractional inhibitory concentration (SFIC) and an isobologram method.
RESULTS
The SFIC values of the three-different-ratio combinations between Tet and Dox ranged from 0.14 to 0.38 for MCF-7, and 0.10 to 0.29 for MCF-7/Dox; those of Tet-Vin combinations ranged from 0.21 to 0.37 for KB, and 0.32 to 0.63 for KBV200. All the SFIC values of the combination between Tet and Dox or Vin were less than 1.0 when the 3 ratios of the 2 drugs in combination were used, and the shapes of isobolic curves obtained from the combination were concave.
CONCLUSION
The interaction between Tet and Dox or Vin against the human cancer cells was markedly synergistic.
目的
研究粉防己碱（Tet）与阿霉素（Dox）或长春新碱（Vin）联合应用对人乳腺癌细胞系MCF - 7和MCF - 7/Dox或人鼻咽癌细胞系KB和KBV200的体外相互作用。
方法
采用四氮唑盐（MTT）法测定单药及Tet与Dox或Vin联合用药的抗癌活性。通过联合抑制浓度分数和（SFIC）值及等效线图法评估Tet与Dox或Vin之间的相互作用。
结果
Tet与Dox不同比例联合用药时，MCF - 7的SFIC值范围为0.14至0.38，MCF - 7/Dox为0.10至0.29；Tet与Vin联合用药时，KB的SFIC值范围为0.21至0.37，KBV200为0.32至0.63。当两种药物按三种比例联合使用时，Tet与Dox或Vin联合用药的所有SFIC值均小于1.0，联合用药所得等效线图呈凹形。
结论
Tet与Dox或Vin联合应用对人癌细胞具有明显的协同作用。
Zotero 插件