离子载体X537A诱导大鼠离体肥大细胞释放组胺的机制。II. 细胞体积增加与组胺释放之间的关系。
The mechanism of histamine release induced by the ionophore X537A from isolated rat mast cells. II. The relationship between increase of cell volume and histamine release.
作者信息
Diamant B, Kazimierczak W, Patkar S A
出版信息
Agents Actions. 1978 Jun;8(4):315-23. doi: 10.1007/BF01968610.
The ionophore X537A induced swelling of isolated rat mast cells parallel to histamine release. Both actions were depressed by extracellular calcium and BSA, temperatures below 37 degrees C, NEM, PMSF, and TTX, and were enhanced by high potassium and pretreatment of the cells with ATP. DSCG, theophylline, and DFP enhanced the histamine release noted after 10 min of incubation without influencing the swelling action of X537A. The swelling action could not be separated from histamine release and it is suggested that it might be inherent in the mechanism of secretion induced by X537A. The present results further distinguish histamine release induced by the two ionophores X537A and A23187.
离子载体X537A可引起离体大鼠肥大细胞肿胀，且与组胺释放平行。这两种作用均受到细胞外钙、牛血清白蛋白、低于37摄氏度的温度、N-乙基马来酰胺（NEM）、苯甲基磺酰氟（PMSF）和河豚毒素（TTX）的抑制，而高钾以及用三磷酸腺苷（ATP）对细胞进行预处理则可增强这两种作用。二丁酰环磷腺苷钙（DSCG）、茶碱和二异丙基氟磷酸（DFP）可增强孵育10分钟后观察到的组胺释放，但不影响X537A的肿胀作用。肿胀作用与组胺释放无法分离，提示其可能是X537A诱导的分泌机制所固有的。目前的结果进一步区分了由两种离子载体X537A和A23187诱导的组胺释放。
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