Reuss M, Doessereck C
Institut für Bioverfahrenstechnik, Universität Stuttgart, Germany.
Ann N Y Acad Sci. 1994 May 2;721:428-39. doi: 10.1111/j.1749-6632.1994.tb47414.x.
Conjugation between two Pseudomonas putida species has been studied. Special emphasis has been given to the design of tools for better defined and reproducible experimental conditions. This is an essential prerequisite for any kinetic analysis of the phenomenon. The experimental approach suggested in the paper is a three-stage continuous fermentation, where donor and recipient strains can be cultivated at predefined steady-state growth rates, and conjugation takes place at steady-state conditions in the third stage. For the first time a remarkable influence of agitative speed on conjugation has been experimentally observed.
对两种恶臭假单胞菌之间的接合作用进行了研究。特别强调了为更好地定义和可重复的实验条件而设计工具。这是对该现象进行任何动力学分析的必要前提。本文提出的实验方法是三阶段连续发酵，其中供体和受体菌株可以在预定义的稳态生长速率下培养，并且在第三阶段在稳态条件下发生接合作用。首次通过实验观察到搅拌速度对接合作用有显著影响。
