Wieczorek A, Michalski H
Faculty of Process and Environmental Engineering, Bioprocess Engineering Department, Technical University of Lódź, Poland.
FEMS Microbiol Rev. 1994 May;14(1):69-74. doi: 10.1111/j.1574-6976.1994.tb00075.x.
Continuous fermentation by a highly flocculant strain of the yeast Saccharomyces cerevisiae was carried out in a tower fluidized-bed bioreactor. The synthetic and molasses media with a total sugar concentration of 17% (w/v) were used for fermentation. Different dilution rates were tested. Stable cell densities of 50 kg m-3 (dry weight) were maintained for all dilution rates. The ethanol productivity was increasing linearly with dilution rates up to 15-20 kg m-3 h-1. Aeration of the culture stabilized flocculating activity and viability of yeast and also permitted long-term operation of the bioreactor.
利用酿酒酵母的一种高絮凝性菌株在塔式流化床生物反应器中进行连续发酵。使用总糖浓度为17%(w/v)的合成培养基和糖蜜培养基进行发酵。测试了不同的稀释率。对于所有稀释率,均保持了50 kg m-3(干重)的稳定细胞密度。乙醇生产率随稀释率线性增加,最高可达15 - 20 kg m-3 h-1。培养物的通气稳定了酵母的絮凝活性和活力,还使生物反应器能够长期运行。