Sheoran A, Yadav B S, Nigam P, Singh D
Department of Microbiology, CCS Haryana Agricultural University, Hisar, India.
J Basic Microbiol. 1998;38(2):123-8. doi: 10.1002/(sici)1521-4028(199805)38:2<123::aid-jobm123>3.0.co;2-9.
A fermentation system for the continuous ethanol production from sucrose and molasses using calcium alginate immobilized Saccharomyces cerevisiae strain HAU-1 has been optimised. Immobilization of active yeast cells (30%, w/v) was accomplished in 1.5% calcium alginate and these yeast-beads were employed for ethanol production in a vertical column reactor. High ethanol productivity was achieved with a medium containing 10%, w/v sucrose at a dilution rate of 0.20 h-1. Alginate beads containing 30-50% yeast cell--mass resulted in high productivity 60.4 g l-1 h-1). Sugarcane molasses containing 15% total sugars was fermented in column reactor to produce 6.0%, v/v ethanol in continuous system. These yields could be improved to 7.0% with overall 11% increase in fermentation--efficiency with the use of acid-clarified sugar cane molasses medium.
一种使用海藻酸钙固定化酿酒酵母菌株HAU-1从蔗糖和糖蜜中连续生产乙醇的发酵系统已得到优化。将活性酵母细胞（30%，w/v）固定在1.5%的海藻酸钙中，这些酵母珠用于在垂直柱式反应器中生产乙醇。在稀释率为0.20 h-1的情况下，使用含10%（w/v）蔗糖的培养基可实现高乙醇生产率。含有30 - 50%酵母细胞质量的海藻酸珠可实现高生产率（60.4 g l-1 h-1）。在柱式反应器中，对含有15%总糖的甘蔗糖蜜进行发酵，在连续系统中生产6.0%（v/v）的乙醇。使用酸澄清甘蔗糖蜜培养基，发酵效率总体提高11%，这些产量可提高到7.0%。
