Ghosh P, Singh U N, Das S
Department of Biophysics, University of Delhi South Campus, India.
Biochem Mol Biol Int. 1994 Jan;32(1):149-58.
We examine the functioning of isolated rat liver gap junction protein after reconstituting into unilamellar liposomes with the help of fluorescence-efflux assay. Monitoring of the dequenching of a fluorescence marker 5, (6) CF due to its efflux from proteoliposomes into extra-liposomal space via its passage through reconstituted gap junctional channels has been performed. The efflux of dye from intra-liposomal to extra-liposomal compartment (both final release or Feq and initial rate or IR) was found dependent on gap junction concentrations. The opening and closing of reconstituted gap junctional channels were found influenced by pH and osmolarity of the extra-liposomal solution.
我们借助荧光外流测定法，研究了重组到单层脂质体中的离体大鼠肝脏间隙连接蛋白的功能。通过荧光标记物5, (6) CF经重组间隙连接通道从蛋白脂质体外流到脂质体外空间，监测其荧光淬灭的解除。发现染料从脂质体内隔室到脂质体外隔室的外流（最终释放量或Feq和初始速率或IR）取决于间隙连接的浓度。还发现重组间隙连接通道的开闭受脂质体外溶液的pH值和渗透压的影响。
