Kam Y, Kim D Y, Koo S K, Joe C O
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
Biochim Biophys Acta. 1998 Jul 17;1372(2):384-8. doi: 10.1016/s0005-2736(98)00075-3.
Gap junction channels reconstituted in liposomes provide a pathway for the transfer of second messengers. Gap junction channels were formed in the artificial unilamellar liposomes using immunoaffinity-purified connexin 43 gap junction protein from rat brain. Sucrose-permeable and -impermeable liposomes were separated on the basis of sucrose permeability in the iso-osmolar sucrose density gradient. The liposomes permeable to sucrose were also permeable to a communicating dye molecule, Lucifer yellow. In the present study, we examined the transfer of second messengers through the connexin 43 channels reconstituted in liposomes and first report the direct evidence that the gap junction channels are permeable to second messengers including adenosine 3',5'-cyclic phosphate and inositol 1,4,5-trisphosphate.
重建于脂质体中的间隙连接通道为第二信使的传递提供了一条途径。利用从大鼠脑中通过免疫亲和纯化得到的连接蛋白43间隙连接蛋白，在人工单层脂质体中形成间隙连接通道。根据等渗蔗糖密度梯度中的蔗糖渗透性，将可渗透蔗糖和不可渗透蔗糖的脂质体分离。可渗透蔗糖的脂质体也可渗透一种通讯染料分子，荧光素黄。在本研究中，我们检测了第二信使通过重建于脂质体中的连接蛋白43通道的传递情况，并首次报告了间隙连接通道可渗透包括3',5'-环磷酸腺苷和肌醇1,4,5-三磷酸在内的第二信使的直接证据。
