人红细胞膜流动性对其制备方法的依赖性。
Dependence of fluidity of human erythrocyte membranes on their methods of preparation.
作者信息
Au K S
机构信息
Department of Biochemistry, University of Hong Kong.
出版信息
Biochem Mol Biol Int. 1994 Jan;32(1):49-53.
PMID:8012289
Abstract
At intermediate temperatures, erythrocyte membranes prepared under isotonic condition were found to be more fluid than membranes prepared under hypotonic condition. Below 17 degrees C and above 30 degrees C, however, no difference in fluidity was observed. The lower steady state diphenylhexatriene fluorescence anisotropy value observed for isotonic membranes was not due to the use of saponin in their preparation but to the loss of membrane-associated proteins extractable by isotonic buffered saline.
摘要
在中等温度下，发现在等渗条件下制备的红细胞膜比在低渗条件下制备的膜流动性更强。然而，在17摄氏度以下和30摄氏度以上，未观察到流动性有差异。等渗膜较低的稳态二苯基己三烯荧光各向异性值并非由于制备过程中使用了皂角苷，而是由于等渗缓冲盐水可提取的膜相关蛋白的丢失。
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