[荧光各向异性——一种表征膜流动性的参数]
[Fluorescence anisotropy--a parameter for characterizing membrane fluidity].
作者信息
Miron S, Rusu V
机构信息
Catedra de Biofizică şi Medicină Nucleară, Facultatea de Medicină, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
出版信息
Rev Med Chir Soc Med Nat Iasi. 1995 Jul-Dec;99(3-4):115-20.
The lipophilic fluorescent probe diphenylhexatriene (DPH) has been shown previously to behave as a marker of plasma membrane in living cell systems, and it is therefore been widely used in membrane fluidity studies via fluorescence anisotropy measurements. The anisotropic coefficient, which is inversely related to the rotational motion of the probe in membrane phospholipids, was significantly higher at 37 degrees C than at 23 degrees C for 9 series of red blood cells ghosts obtained from three healthy subjects. We also have studied the importance of the nature of two different polaroid films which permits the observation of fluorescence polarization.
亲脂性荧光探针二苯基己三烯（DPH）此前已被证明在活细胞系统中可作为质膜的标志物，因此它已通过荧光各向异性测量被广泛用于膜流动性研究。对于从三名健康受试者获得的9组红细胞血影，与探针在膜磷脂中的旋转运动呈反比关系的各向异性系数在37℃时显著高于23℃时。我们还研究了两种不同偏振片的性质对于观察荧光偏振的重要性。
Zotero 插件