枯草芽孢杆菌中甘油调节子的glpT和glpQ基因。

The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis.

作者信息

Nilsson R P, Beijer L, Rutberg B

机构信息

Department of Microbiology, Lund University, Sweden.

出版信息

Microbiology (Reading). 1994 Apr;140 ( Pt 4):723-30. doi: 10.1099/00221287-140-4-723.

DOI:10.1099/00221287-140-4-723

PMID:8012593

Abstract

The cloning of the Bacillus subtilis glpT and glpQ genes and their nucleotide sequences are reported. Analysis of mRNA indicates that glpT and glpQ constitute one operon which is transcribed from a sigma A type promoter. The steady state amount of glpTQ mRNA is increased in cells grown in the presence of glycerol 3-phosphate. The 5' untranslated leader sequence of glpTQ mRNA contains an inverted repeat which shows sequence similarity to repeats present in the leader sequences of glpFK and glpD transcripts. These repeats seem therefore to be essential control elements for all B. subtilis glp genes.

摘要

已报道了枯草芽孢杆菌glpT和glpQ基因的克隆及其核苷酸序列。对信使核糖核酸（mRNA）的分析表明，glpT和glpQ构成一个操纵子，该操纵子从σA类型启动子转录。在存在3-磷酸甘油的情况下生长的细胞中，glpTQ mRNA的稳态量增加。glpTQ mRNA的5'非翻译前导序列包含一个反向重复序列，该序列与glpFK和glpD转录本前导序列中存在的重复序列具有序列相似性。因此，这些重复序列似乎是所有枯草芽孢杆菌glp基因的重要控制元件。