Lutz-Ostertag Y
Arch Anat Histol Embryol. 1976;59:5-18.
Right and left müllerian ducts of 6 to 9 days old chick embryos, 7 to 9 days old quail embryos and left differentiated ducts of chick embryos to 10 to 19 days of development are explanted on medium with testosterone. In "in vitro" culture, this hormone is able to provoke regressive phenomenons and the complete disapearance of müllerian epithelium; only a mensenchyme string subsists similarly to that observed "in ovo" during the involution of these gonoducts in the male embryos. The müllerian epithelial cells of the 19 days old female embryos undergo a quick autolysis. The action is selective, neither the Wolffian duct nor the mesonephros are attaqued.
将6至9日龄鸡胚、7至9日龄鹌鹑胚的左右苗勒管以及发育至10至19日龄鸡胚的左分化管接种于含有睾酮的培养基上。在“体外”培养中,这种激素能够引发退化现象以及苗勒上皮的完全消失;仅留存一条间充质索,类似于在雄性胚胎中这些生殖管道退化期间“在体内”观察到的情况。19日龄雌性胚胎的苗勒上皮细胞会迅速自溶。这种作用具有选择性,中肾管和中肾均未受到影响。