Watanabe K, Tullis J L
Am J Med Sci. 1978 May-Jun;275(3):337-44. doi: 10.1097/00000441-197805000-00011.
Ristocetin, at relatively low concentrations (1.0 mg/ml-1.5 mg/ml), can selectively precipitate fibrin monomers and fibrin degradation products (fdp) from plasma without effect on fibrinogen or fibrinogen degradation products (FDP). 125I-labeled fibrin monomers and fibrin degradation products were precipitated by ristocetin when their plasma concentrations were greater than 0.25 microgram/ml and 50 microgram/ml, respectively. In order to obtain a visible precipitated, 2 microgram/ml of fibrin monomers of 50 to 100 microgram/ml of fibrin degradation products were necessary. These effects were optimally observed under the following conditions: (1) temperature, 20 C to 37 C; (2) pH, 7.0 to 7.5; and (3) incubation time, 15 to 60 minutes. Late-fibrin degradation products are approximately eight times less sensitive to ristocetin-induced precipitation than early-fibrin degradation products. Plasma medium is essential for the differentiation of fibrin monomers and fibrin degradation products from fibrinogen and fibrinogen degradation products by ristocetin. These results suggest that the specific detection of fibrin monomers and fibrin degradation products in plasma may be easily performed by ristocetin.
在相对较低浓度（1.0毫克/毫升 - 1.5毫克/毫升）时，瑞斯托菌素可从血浆中选择性沉淀纤维蛋白单体和纤维蛋白降解产物（fdp），而不影响纤维蛋白原或纤维蛋白原降解产物（FDP）。当血浆中125I标记的纤维蛋白单体和纤维蛋白降解产物的浓度分别大于0.25微克/毫升和50微克/毫升时，可被瑞斯托菌素沉淀。为了获得可见沉淀，需要2微克/毫升的纤维蛋白单体或50至100微克/毫升的纤维蛋白降解产物。在以下条件下可最佳观察到这些效应：（1）温度，20℃至37℃；（2）pH值，7.0至7.5；（3）孵育时间，15至60分钟。晚期纤维蛋白降解产物对瑞斯托菌素诱导沉淀的敏感性比早期纤维蛋白降解产物低约八倍。血浆介质对于瑞斯托菌素区分纤维蛋白单体和纤维蛋白降解产物与纤维蛋白原和纤维蛋白原降解产物至关重要。这些结果表明，通过瑞斯托菌素可轻松实现血浆中纤维蛋白单体和纤维蛋白降解产物的特异性检测。
