Santos-Montes A, Gonzalo-Lumbreras R, Gasco-Lopez A I, Izquierdo-Hornillos R
Departamento de Química Analítica, Facultad de Química, Universidad Complutense, Madrid, Spain.
J Chromatogr. 1994 Jan 14;652(1):83-9. doi: 10.1016/0378-4347(93)e0396-8.
Optimization of the main variables that affect solvent and solid-phase extraction processes, using disposable C18 cartridges and the non-ionic polymeric resin Serdolit AD-2, of human urine containing natural and synthetic corticoids is described. The data were obtained from different HPLC separations of these compounds using calibration graphs obtained before and after extraction of these compounds. The procedures, including sample preconcentration, showed efficiencies over 90%. NaCl was used to avoid emulsion formation in solvent extraction. The results achieved using solvent and solid-phase extraction are discussed.
描述了使用一次性C18柱和非离子聚合物树脂Serdolit AD-2对含有天然和合成皮质类固醇的人尿进行溶剂萃取和固相萃取过程中主要变量的优化。这些数据来自于这些化合物的不同高效液相色谱分离，使用了在萃取前后获得的校准曲线。包括样品预浓缩在内的方法显示出超过90%的效率。使用氯化钠来避免溶剂萃取中形成乳液。讨论了使用溶剂萃取和固相萃取所获得的结果。
