Neuhüser T, Sorge D, Stay B, Hoffmann K H
Abteilung Allgemeine Zoologie, Universität Ulm, Germany.
J Comp Physiol B. 1994;164(1):23-31. doi: 10.1007/BF00714567.
Brain-retrocerebral complexes of female crickets, Gryllus bimaculatus and Acheta domesticus, treated with antibody to allatostatin-1 from a cockroach, Diploptera punctata, show extensive immunoreactivity. The results suggest that allostatins or allatostatin-like molecules are produced in neurosecretory cells of the brain and are delivered to the corpora allata through nervous connections and/or via haemolymph. Radiochemical measurements of juvenile hormone III biosynthesis by isolated corpora cardiaca-corpora allata complexes from adult G. bimaculatus have been used to demonstrate an in vitro sensitivity of these glands to allatostatin-1 from D. punctata. Allatostatin-1 is a relatively potent inhibitor of juvenile hormone III biosynthesis in corpora allata of both young adult females and males. In glands taken from 3-day virgin females, 50% inhibition of hormone biosynthesis is reached at ca. 3 nmol.l-1 allatostatin-1. The inhibitory action of allatostatin-1 is rapid, dose-dependent and reversible. Addition of 200 mumol.l-1 farnesol to the incubation medium prevents inhibition of juvenile hormone III biosynthesis by allatostatin-1. Juvenile hormone III biosynthesis by isolated corpora allata of 3-day female house crickets, A. domesticus, is also susceptible to inhibition by 1 mumol.l-1 allatostatin-1.
用来自蟑螂双斑大蠊的抑咽侧体素 -1抗体处理的双斑蟋蟀和家蟋蟀雌性的脑 - 后脑复合体显示出广泛的免疫反应性。结果表明，抑咽侧体素或类抑咽侧体素分子在脑的神经分泌细胞中产生，并通过神经连接和/或通过血淋巴传递到咽侧体。通过分离成年双斑蟋蟀的心侧体 - 咽侧体复合体对保幼激素III生物合成进行放射化学测量，已用于证明这些腺体对来自双斑大蠊的抑咽侧体素 -1的体外敏感性。抑咽侧体素 -1是成年雌性和雄性咽侧体中保幼激素III生物合成的相对有效抑制剂。在取自3日龄未交配雌性的腺体中，在约3 nmol·l-1的抑咽侧体素 -1时达到激素生物合成的50％抑制。抑咽侧体素 -1的抑制作用迅速、剂量依赖性且可逆。向孵育培养基中添加200 μmol·l-1法尼醇可防止抑咽侧体素 -1对保幼激素III生物合成的抑制。3日龄雌性家蟋蟀分离的咽侧体的保幼激素III生物合成也易受1 μmol·l-1抑咽侧体素 -1的抑制。
