Rankin S M, Stay B, Chan K, Jackson E S
Department of Biology, Allegheny College, Meadville, Pennsylvania, USA.
Arch Insect Biochem Physiol. 1998;38(4):155-65. doi: 10.1002/(SICI)1520-6327(1998)38:4<155::AID-ARCH1>3.0.CO;2-Q.
A monoclonal antibody to allatostatin I of the cockroach Diploptera punctata was used to demonstrate the presence of allatostatin-immunoreactive cells and fiber tracts in the neuroendocrine system of the earwig Euborellia annulipes. The corpora cardiaca cells were not immunoreactive, nor were the neurosecretory endings of fiber tracts from the brain to the corpora cardiaca. No immunoreactive material was detected in the corpus allatum, although the corpus allatum contained neurosecretory endings, and some cells of the brain, including medial and lateral protocerebral cells, showed immunoreactivity. In addition, the recurrent and esophageal nerves were allatostatin-positive. The last abdominal ganglion contained immunoreactive somata, and immunoreactive axons of the proctodeal nerve innervated the rectum, anterior intestine, and posterior midgut. We did not detect reactive endocrine cells in the midgut. Allatostatin I at concentrations of 10(-5) and 10(-7) M did not inhibit juvenile hormone biosynthesis by E. annulipes corpora allata in vitro. This was true for glands of low activity from 2-day females and brooding females, as well as for relatively high activity glands from 10-day females. In contrast, 10(-7) M allatostatin I significantly and reversibly decreased hindgut motility. Motility was decreased in hindguts of high endogenous motility from 2-day females and in those of relatively low activity from brooding females. These results support the notion that a primary function of allatostatin might be to reduce gut motility.
一种针对点刻原伪蝎蛉咽侧体抑制素I的单克隆抗体被用于证明在环带真地鳖神经内分泌系统中存在咽侧体抑制素免疫反应性细胞和纤维束。心侧体细胞无免疫反应性，从脑到心侧体的纤维束的神经分泌末梢也无免疫反应性。在咽侧体中未检测到免疫反应性物质，尽管咽侧体含有神经分泌末梢，并且脑的一些细胞，包括中脑和侧脑原细胞，显示出免疫反应性。此外，返神经和食管神经呈咽侧体抑制素阳性。最后一个腹神经节含有免疫反应性胞体，直肠神经的免疫反应性轴突支配直肠、前肠和中肠后部。我们在中肠中未检测到反应性内分泌细胞。10^(-5)和10^(-7) M浓度的咽侧体抑制素I在体外并未抑制环带真地鳖咽侧体的保幼激素生物合成。对于2日龄雌性和育雏雌性低活性腺体以及10日龄雌性相对高活性腺体而言都是如此。相比之下，10^(-7) M的咽侧体抑制素I显著且可逆地降低了后肠运动性。2日龄雌性高内源性运动性后肠以及育雏雌性相对低活性后肠的运动性均降低。这些结果支持了咽侧体抑制素的主要功能可能是降低肠道运动性这一观点。
