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埃及伊蚊和白纹伊蚊中促咽侧体素以及抑咽侧体素-A和-C的免疫染色。

Immunostaining for allatotropin and allatostatin-A and -C in the mosquitoes Aedes aegypti and Anopheles albimanus.

作者信息

Hernández-Martínez Salvador, Li Yiping, Lanz-Mendoza Humberto, Rodríguez Mario H, Noriega Fernando G

机构信息

Department of Biological Sciences, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA.

出版信息

Cell Tissue Res. 2005 Jul;321(1):105-13. doi: 10.1007/s00441-005-1133-5. Epub 2005 May 21.

DOI:10.1007/s00441-005-1133-5
PMID:15909164
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2647714/
Abstract

Confocal laser-scanning microscopy was used to carry out a comparative study of the immunostaining for three families of neuropeptides, viz., allatostatin-A (AS-A), allatostatin-C (AS-C) and allatotropin (AT), in adult female mosquitoes of Aedes aegypti and Anopheles albimanus. The specific patterns of immunostaining for each of the three peptides were similar in both species. The antisera raised against AT, AS-A, and AS-C revealed intense immunoreactivity in the cells of each protocerebral lobe of the brain and stained cells in each of the ventral ganglia and neuronal projections innervating various thoracic and abdominal tissues. Only the AS-A antiserum labeled immunoreactive endocrine cells in the midgut. The distribution of the peptides supports the concept that they play multiple regulatory roles in both species.

摘要

利用共聚焦激光扫描显微镜对埃及伊蚊和白纹伊蚊成年雌性蚊子中三种神经肽家族，即抑咽侧体素 -A（AS-A）、抑咽侧体素 -C（AS-C）和促咽侧体素（AT）的免疫染色进行了比较研究。两种蚊子中这三种肽各自的免疫染色特异性模式相似。针对AT、AS-A和AS-C产生的抗血清在大脑每个原脑叶的细胞中显示出强烈的免疫反应，并对每个腹神经节以及支配各种胸部和腹部组织的神经突起中的细胞进行了染色。只有AS-A抗血清标记了中肠中的免疫反应性内分泌细胞。这些肽的分布支持了它们在这两个物种中都发挥多种调节作用的观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/aaef5e94087a/nihms93609f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/a5c656ac4247/nihms93609f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/b20931aeb44e/nihms93609f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/df67e7456047/nihms93609f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/aaef5e94087a/nihms93609f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/a5c656ac4247/nihms93609f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/7068a523e8ed/nihms93609f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/5a2792b72f4d/nihms93609f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/b20931aeb44e/nihms93609f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/df67e7456047/nihms93609f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2062/2647714/aaef5e94087a/nihms93609f6.jpg

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