Sonaka I, Futami Y, Maki T
Central Research Laboratories, Ajinomoto Co., Inc., Yokohama, Japan.
J Gerontol. 1994 Jul;49(4):B157-61. doi: 10.1093/geronj/49.4.b157.
Effects of aging and dietary protein on the L-arginine-nitric oxide (Arg-NO) pathway and the progress of chronic nephropathy were examined. At 6-7 months of age, 10 male Fischer 344 rats were fed a 23% protein diet until 24 or 25 months of age, and another 10 were fed a 12% protein diet until that age. Twenty male Fischer 344 rats that were fed the 23% protein diet from 6 to 8 months of age were used as a control. Urinary excretion of nitrite/nitrate (NOx) at the age of 24 months in the 23% protein group was remarkably decreased, whereas in the 12% protein group, urinary NOx remained comparable to that of the control. Histological examination revealed that chronic nephropathy was highly progressive in the 23% protein group, accompanied by lowered renal function, but these changes were obviously suppressed in the 12% protein group. These results suggest that an age-related decrease in the synthesis of NO could be associated with the progress of chronic nephropathy.
研究了衰老和膳食蛋白质对L-精氨酸-一氧化氮（Arg-NO）途径及慢性肾病进展的影响。6至7月龄时，10只雄性Fischer 344大鼠喂食23%蛋白质饮食直至24或25月龄，另外10只喂食12%蛋白质饮食直至该年龄。20只在6至8月龄时喂食23%蛋白质饮食的雄性Fischer 344大鼠用作对照。23%蛋白质组在24月龄时尿中亚硝酸盐/硝酸盐（NOx）排泄显著降低，而12%蛋白质组尿中NOx与对照组相当。组织学检查显示，23%蛋白质组慢性肾病进展严重，伴有肾功能降低，但这些变化在12%蛋白质组中明显受到抑制。这些结果表明，与年龄相关的一氧化氮合成减少可能与慢性肾病的进展有关。
