Huang F L, Zhuo H, Sinclair C, Goldstein M E, McCabe J T, Helke C J
Department of Pharmacology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814.
Brain Res Mol Brain Res. 1994 Mar;22(1-4):290-8. doi: 10.1016/0169-328x(94)90057-4.
Visceral sensory neurons of the glossopharyngeal and vagus nerves are located in the petrosal and nodose ganglia, respectively. Our previous studies showed that peripheral axotomy which removes afferent input to visceral sensory perikarya decreased the number of calcitonin gene-related peptide (CGRP)-immunoreactive (ir) neurons in the petrosal but not the nodose ganglion. To evaluate axotomy-induced changes in CGRP mRNA expression, we used in situ hybridization histochemistry with 35S-labeled oligonucleotide probes. CGRP mRNA-containing neurons were studied 1, 3, 7 and 14 days after peripheral deafferentation of the left nodose and petrosal ganglia via transection of the left cervical vagus, superior laryngeal, glossopharyngeal and carotid sinus nerves. The numbers of CGRP mRNA-containing neurons in the deafferented petrosal ganglion were significantly reduced at 3, 7 and 14 days compared to either intact or sham-operated control ganglia. However, the density of hybridization product in the positively-labeled petrosal ganglion cells was not significantly changed. The numbers of CGRP mRNA-containing neurons in the deafferented nodose ganglion were significantly reduced at 3 and 7 days. These data suggest that axotomy-induced changes in CGRP-ir neurons of the petrosal ganglion correlate with changes in CGRP mRNA and probably result from altered CGRP gene expression. In addition, in situ hybridization histochemistry revealed changes in CGRP neurons of the nodose ganglion which were not apparent with immunocytochemistry.
舌咽神经和迷走神经的内脏感觉神经元分别位于岩神经节和结状神经节。我们之前的研究表明，切断外周轴突以消除内脏感觉神经元胞体的传入输入，会减少岩神经节中降钙素基因相关肽（CGRP）免疫反应性（ir）神经元的数量，但不会减少结状神经节中的此类神经元数量。为了评估轴突切断术引起的CGRP mRNA表达变化，我们使用了35S标记的寡核苷酸探针进行原位杂交组织化学研究。通过切断左侧颈迷走神经、喉上神经、舌咽神经和颈动脉窦神经，对左侧结状神经节和岩神经节进行外周去传入后1、3、7和14天，研究含CGRP mRNA的神经元。与完整或假手术对照神经节相比，去传入的岩神经节中含CGRP mRNA的神经元数量在3、7和14天时显著减少。然而，阳性标记的岩神经节细胞中杂交产物密度没有显著变化。去传入的结状神经节中含CGRP mRNA的神经元数量在3和7天时显著减少。这些数据表明，轴突切断术引起的岩神经节CGRP-ir神经元变化与CGRP mRNA变化相关，可能是由于CGRP基因表达改变所致。此外，原位杂交组织化学揭示了结状神经节CGRP神经元的变化，而免疫细胞化学未显示出这些变化。
