Engelberts I, Francot G J, Leunissen K M, Haenen B, Ceska M, van der Linden C J, Buurman W A
Department of Surgery, University of Limburg, Maastricht, The Netherlands.
Nephron. 1994;66(4):396-403. doi: 10.1159/000187853.
The influence of blood-membrane interaction on human peripheral blood monocyte tumor necrosis factor-alpha (TNF), interleukin-6 (IL-6), and interleukin-8 (IL-8) secretion was measured during hemodialysis of end-stage renal disease patients by in vitro stimulation of whole blood with lipopolysaccharide. Monocyte TNF and IL-6 secretion in vitro was reduced 30 min after start of dialysis session. In contrast, cellular IL-8 secretion did not change during hemodialysis. Comparison of the results of three different membranes indicates that the bioincompatibility of the dialysis membrane was reflected in both leukocytopenia and reduction of cellular TNF secretion. During treatment of normal whole blood in an ex vivo dialysis closed-loop circuit, the ability of monocytes to release TNF, IL-6, and IL-8 in vitro remained constant. This indicates that the reduced IL-6 and TNF secretion during standard hemodialysis was not due to a direct effect of contact between dialysis membranes and monocytes, but rather was a result of redistribution within the patients' leukocyte pool.
在终末期肾病患者血液透析期间，通过用脂多糖体外刺激全血，测定了血膜相互作用对人外周血单核细胞肿瘤坏死因子-α（TNF）、白细胞介素-6（IL-6）和白细胞介素-8（IL-8）分泌的影响。透析开始30分钟后，体外单核细胞TNF和IL-6分泌减少。相比之下，血液透析期间细胞IL-8分泌没有变化。对三种不同膜的结果进行比较表明，透析膜的生物不相容性体现在白细胞减少和细胞TNF分泌减少两方面。在体外透析闭环回路中处理正常全血时，单核细胞体外释放TNF、IL-6和IL-8的能力保持恒定。这表明标准血液透析期间IL-6和TNF分泌减少并非透析膜与单核细胞之间接触的直接作用所致，而是患者白细胞池内重新分布的结果。
