Saito M, Broderick G A, Hypolite J A, Levin R M
Division of Urology, University of Pennsylvania, School of Medicine, Philadelphia.
Pharmacology. 1994 May;48(5):335-40. doi: 10.1159/000139197.
Pharmacologic and behavioral effects of alcohol on male sexual activity have long been controversial. Among the varied effects of alcohol, it has been described that ethanol reduces nitric oxide production. Appreciating the importance of nitric oxide-mediated regulation of corporal smooth muscle, this study was designed to investigate the in vitro pharmacologic effect of 1-5% ethanol on rabbit corpus cavernosum function. The results are summarized as follows. In isolated organ bath experiments, basal resting tension of corporal strips was significantly reduced with 3 and 5% ethanol exposure. Relaxation induced by field stimulation over a frequency range of 2-16 Hz was significantly decreased with 3 and 5% ethanol exposure. However, field-stimulated contraction at 32 Hz was reduced by 5% ethanol only. When corporal strips were preincubated with phenylephrine, 3 and 5% ethanol significantly inhibited field stimulated relaxation at all frequencies. Both phasic and tonic contraction induced by phenylephrine was significantly suppressed by 3 and 5% ethanol. KCl-induced contraction was decreased by 5% ethanol. ATP-induced relaxation was significantly enhanced by 1, 3 and 5% ethanol. Bethanechol-induced relaxation was significantly suppressed by 1, 3 and 5% ethanol. Direct nitroprusside-induced relaxation was not affected by any concentration of ethanol administration. These results demonstrated that ethanol had significant effects on both contraction and relaxation of rabbit corpus cavernosum. In general, corporal relaxation mediated through the acetylcholine-L-arginine-nitric oxide pathway was significantly more sensitive to ethanol than either ATP- or nitroprusside-induced relaxation.
酒精对男性性活动的药理和行为影响长期以来一直存在争议。在酒精的多种作用中，已有描述称乙醇会减少一氧化氮的产生。鉴于一氧化氮介导的海绵体平滑肌调节的重要性，本研究旨在调查1 - 5%乙醇对兔海绵体功能的体外药理作用。结果总结如下。在离体器官浴实验中，暴露于3%和5%乙醇时，海绵体条带的基础静息张力显著降低。暴露于3%和5%乙醇时，在2 - 16Hz频率范围内场刺激诱导的舒张显著减少。然而，仅5%乙醇可降低32Hz时的场刺激收缩。当海绵体条带用去氧肾上腺素预孵育时，3%和5%乙醇在所有频率下均显著抑制场刺激舒张。3%和5%乙醇显著抑制去氧肾上腺素诱导的相性和强直性收缩。5%乙醇可降低氯化钾诱导的收缩。1%、3%和5%乙醇显著增强三磷酸腺苷诱导的舒张。1%、3%和5%乙醇显著抑制氨甲酰甲胆碱诱导的舒张。直接给予硝普钠诱导的舒张不受任何浓度乙醇给药的影响。这些结果表明，乙醇对兔海绵体的收缩和舒张均有显著影响。一般来说，通过乙酰胆碱 - L - 精氨酸 - 一氧化氮途径介导的海绵体舒张对乙醇的敏感性明显高于三磷酸腺苷或硝普钠诱导的舒张。
